鞭毛内转运蛋白传递到纤毛基部并组装成序列

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-04-04 DOI:10.1126/sciadv.adr1716

Aniruddha Mitra, Evangelos Gioukakis, Wouter Mul, Erwin J. G. Peterman

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引用次数: 0

摘要

由IFT- b、IFT- a和BBSome亚复合物组成的顺行纤毛内运输（IFT）序列负责将纤毛蛋白运输到纤毛中。IFT亚复合物如何到达纤毛碱基并组装成IFT序列尚不清楚。在这里，我们对秀丽隐杆线虫的化学感觉纤毛进行了定量单分子成像，以揭示IFT亚复合物如何到达基部，在IFT序列中组织，并进入纤毛。我们发现BBSomes通过扩散到达碱基，在那里它们要么与组装IFT序列结合，要么与围绕碱基的膜结合。相比之下，IFT-B和IFT-A通过定向运输到达碱基，最有可能通过停在碱基附近不同位置的囊泡到达。单个亚复合物从囊泡中分离到扩散池中，并与组装序列相结合。我们的研究结果表明，IFT- b首先被纳入IFT序列，然后是IFT- a，最后是BBSomes，这表明IFT序列的组装是一个高度调控的、逐步的过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Delivery of intraflagellar transport proteins to the ciliary base and assembly into trains

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Delivery of intraflagellar transport proteins to the ciliary base and assembly into trains

Anterograde intraflagellar transport (IFT) trains, composed of IFT-B, IFT-A, and BBSome subcomplexes, are responsible for transporting ciliary proteins into the cilium. How IFT subcomplexes reach the ciliary base and assemble into IFT trains is poorly understood. Here, we perform quantitative single-molecule imaging in Caenorhabditis elegans chemosensory cilia to uncover how IFT subcomplexes arrive at the base, organize in IFT trains, and enter the cilium. We find that BBSomes reach the base via diffusion where they either associate with assembling IFT trains or with the membrane surrounding the base. In contrast, IFT-B and IFT-A reach the base via directed transport most likely on vesicles that stop at distinct locations near the base. Individual subcomplexes detach from the vesicles into a diffusive pool and associate to assembling trains. Our results show that IFT-B is first incorporated into IFT trains, followed by IFT-A, and finally BBSomes, indicating that the assembly of IFT trains is a highly regulated, step-wise process.

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.