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IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-06 DOI:10.1016/j.jff.2025.106800

Tianming Yao , Yunus E. Tuncil , Sajal Bhattarai , Manoj Gurung , Lars Bode , Laxmi Yeruva , Stephen R. Lindemann

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引用次数: 0

摘要

人们对母体微RNA（miRNA）和母乳低聚糖（HMO）在影响婴儿肠道细菌方面的相互作用仍然知之甚少。本研究调查了牛奶 miRNA 如何影响以 HMOs 和乳糖（1% w/v）为底物培养的婴儿双歧杆菌的生长动态和基因表达。用人乳 miRNA 或合成模拟物（500 毫微克/毫升）对菌株进行体外培养。婴儿畸形芽孢杆菌在 HMOs 上的生长速度（11.23 小时达到峰值）明显快于乳糖（12.75 小时），但 miRNA 对这两种底物的生长影响极小。转录组分析进一步显示，miRNA 处理后的基因表达差异有限，HMO-miRNA 组只有 11 个基因表达过高，其中没有一个可能影响新陈代谢。相比之下，底物类型导致 1986 个基因的表达发生变化，这些基因涉及 ABC 转运体、氨基酸生物合成、嘌呤和碳水化合物代谢。这项研究强调了 HMOs 比 miRNAs 对婴儿肠杆菌生物学的影响更大，尽管两者在牛奶中都很丰富。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effect of maternal miRNAs and milk oligosaccharides on regulating the growth behavior of Bifidobacterium longum subsp. infantis

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Effect of maternal miRNAs and milk oligosaccharides on regulating the growth behavior of Bifidobacterium longum subsp. infantis

The interplay between maternal microRNAs (miRNAs) and human milk oligosaccharides (HMOs) in influencing infant gut bacteria remains poorly understood. This study investigated how milk miRNAs impact the growth dynamics and gene expression of Bifidobacterium infantis cultured on HMOs and lactose as substrates (1 % w/v). The strain was cultured in vitro with either human milk miRNAs or synthetic mimics (500 ng/mL). B. infantis grew significantly faster on HMOs (plateau at 11.23 h) than lactose (12.75 h), but miRNAs showed minimal effects on growth in both substrates. Transcriptome analysis further showed limited differential gene expression upon miRNA treatments, with only 11 overexpressed genes in the HMO-miRNA group, none likely affecting metabolism. In contrast, substrate type drove expression changes in 1986 genes, involving in ABC transporters, amino acid biosynthesis, purine, and carbohydrate metabolism. This research highlighted the stronger effects of HMOs than miRNAs on B. infantis biology, despite both being abundant in milk.

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.