Fertilization and the fast block to polyspermy in the African Clawed Frog, Xenopus laevis: A historical perspective.

The African clawed frog, Xenopus laevis, has long been a model organism for studying fertilization due to its large and abundant eggs that are easily manipulated and rapidly undergo embryonic development. Research on this model organism has provided significant insights into the mechanisms that ensure successful fertilization, including the prevention of polyspermy. Polyspermy, the fertilization of an egg by multiple sperm, poses a significant threat to successful embryonic development in most sexually reproducing animals. To counter this, eggs have evolved mechanisms known as polyspermy blocks, which prevent additional sperm from entering once fertilization has occurred. This review focuses on fertilization research in general, and specifically on studies of the fast block to polyspermy in X. laevis. We trace key discoveries and experimental advancements that have shaped our current understanding. Indeed, studies on X. laevis have revealed that fertilization triggers a depolarization of the egg membrane mediated by an efflux of Cl^- through the Ca²⁺-activated Cl^- channel TMEM16A, effectively preventing polyspermy. Despite these advances, several questions remain regarding the precise molecular interactions and signaling pathways involved. Continued research on X. laevis promises to uncover further details about the earliest events in embryogenesis and the voltage-dependent mechanisms of fertilization, offering broader insights into reproductive biology across species.