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IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary World Pub Date : 2025-02-01 Epub Date: 2025-02-19 DOI:10.14202/vetworld.2025.397-407

Iwan Sahrial Hamid, Lailatul Muniroh, Salipudin Tasil Maslamama, Rondius Solfaine

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引用次数: 0

摘要

背景和目的：糖尿病肾病（DN）是糖尿病的一种主要并发症，以氧化应激和炎症为特征。传统的治疗方法往往无法阻止其恶化。本研究探讨了 Tithonia diversifolia 氧化锌纳米颗粒乳液（TDNP）通过增强抗氧化和免疫调节机制来缓解 DN 的治疗潜力。本研究旨在评估 TDNP 对链佐菌素（STZ）诱导的 DN 大鼠氧化应激标记物、炎症和胰岛素活性的影响：雄性 Wistar 大鼠（n = 24）分为四组：对照组（生理盐水）、阳性对照组（0.1% 氧化锌悬浮液）、治疗组（100 毫克/公斤体重的 TDNP）和比较组（5 毫克/公斤体重的槲皮素）。使用 STZ 和烟酰胺诱导 DN。评估血糖、肌酐、尿素、γ-谷氨酰转移酶（γ-GT）和高密度脂蛋白胆固醇水平。对氧化应激标志物（超氧化物歧化酶[SOD]、谷胱甘肽过氧化物酶[GPx]）、炎症细胞因子（TNF-α）和免疫组化指标（抗胰岛素、γ干扰素[IFN-γ]）进行了测定。数据分析采用单因素方差分析和 Kruskal-Wallis 检验：结果：与阳性对照组相比，TDNP 治疗明显降低了血糖、肌酐、尿素、γ-GT 和 TNF-α 水平（p ≤ 0.05），同时提高了 SOD、GPx 和抗胰岛素水平。组织病理学分析表明，胰腺和肾脏组织中的坏死和炎症减少。免疫组化显示，TDNP 处理的大鼠抗胰岛素增强，IFN-γ 表达减少，表明免疫调节和氧化应激缓解得到改善：结论：TDNP 具有强大的抗氧化和抗炎作用，能有效改善 DN 的糖代谢和肾功能。这些研究结果表明，TDNP 是一种治疗 DN 的有效药物。

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Effect of feeding Tithonia diversifolia zinc oxide nanoparticle emulsion on glutathione peroxidase and anti-insulin production in diabetic nephropathy Wistar rats.

Background and aim: Diabetic nephropathy (DN) is a major complication of diabetes mellitus characterized by oxidative stress and inflammation. Conventional treatments often fail to prevent its progression. This study investigates the therapeutic potential of Tithonia diversifolia zinc oxide nanoparticle emulsion (TDNP) in mitigating DN by enhancing antioxidant and immunomodulatory mechanisms. This study aimed to evaluate the effect of TDNP on oxidative stress markers, inflammation, and insulin activity in streptozotocin (STZ)-induced DN rats.

Materials and methods: Male Wistar rats (n = 24) were divided into four groups: control (saline), positive control (0.1% zinc oxide suspension), treatment (TDNP at 100 mg/kg body weight), and comparison (quercetin at 5 mg/kg body weight). DN was induced using STZ and nicotinamide. Blood glucose, creatinine, urea, gamma-glutamyl transferase (γ-GT), and high-density lipoprotein cholesterol levels were assessed. Oxidative stress markers (superoxide dismutase [SOD], glutathione peroxidase [GPx]), inflammatory cytokines (TNF-α), and immunohistochemical indicators (anti-insulin, interferon-gamma [IFN-γ]) were measured. Data were analyzed using a one-way analysis of variance and Kruskal-Wallis tests.

Results: TDNP treatment significantly reduced blood glucose, creatinine, urea, γ-GT, and TNF-α levels (p ≤ 0.05), while increasing SOD, GPx, and anti-insulin levels compared with the positive control. Histopathological analysis showed decreased necrosis and inflammation in pancreatic and renal tissues. Immunohistochemistry revealed enhanced anti-insulin and reduced IFN-γ expression in TDNP-treated rats, indicating improved immune regulation and oxidative stress mitigation.

Conclusion: TDNP demonstrates potent antioxidative and anti-inflammatory effects, effectively improving glucose metabolism and kidney function in DN. These findings highlight TDNP as a promising therapeutic agent for managing DN.

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来源期刊

Veterinary World Multiple-

CiteScore

3.60

自引率

12.50%

发文量

317

审稿时长

16 weeks

期刊介绍： Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.