A A P Milton, Aleimo G Momin, K Srinivas, G Bhuvana Priya, P N Gandhale, D M Firake, Samir Das, S Ghatak, A Sen
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引用次数: 0
摘要
背景和目的：单形链球菌是鼠咬热（RBF）和哈弗希尔热（HF）等人畜共患病的病原体。虽然人类感染病例屡见不鲜，但对其在印度啮齿类动物中存在情况的研究却十分有限。本研究旨在检测印度东北部自由生活的箭猪（Bandicota bengalensis 和 Bandicota indica）中的 S. moniliformis，并进行系统发育分析以评估其与全球分离株的遗传关系：从印度梅加拉亚邦的不同环境中捕获了106只Bandicoot（B. bengalensis，n = 76；B. indica，n = 30）。采集粪便和组织样本并进行 DNA 提取。使用针对 16S 核糖体 RNA 基因的物种特异性聚合酶链反应（PCR）对 S. moniliformis 进行分子检测。对阳性扩增子进行测序，使用基本局部比对搜索工具进行分析，并进行系统进化分析：结果：基于 PCR 的检测结果显示，B. indica 的粪便感染率为 3.3%（1/30），B. bengalensis 为 0%。没有组织样本对 S. moniliformis 呈阳性检测。检测到的分离物与之前报告的单形杆菌菌株的序列同一性为 100%，与 Notomytis 链球菌的相似性为 99.63%。系统发育分析将该分离株与全球多个地区的人源和啮齿类动物源 S. moniliformis 株进行了聚类，这表明可能存在人畜共患传播：本研究首次在印度的袋鼬中分子检测到了单形尾柱虫，突显了其人畜共患病的可能性。鉴于其通过啮齿动物叮咬和排泄物污染传播的风险，公共卫生监测至关重要。对于长期发热的患者，尤其是在啮齿动物流行地区，临床医生应考虑RBF和HF。
Detection and phylogenetic analysis of Streptobacillus moniliformis, the causative agent of rat-bite fever and Haverhill fever, in free-living greater bandicoot rats in Northeastern India.
Background and aim: Streptobacillus moniliformis is the causative agent of zoonotic diseases such as rat-bite fever (RBF) and Haverhill fever (HF). While human infections are well-documented, limited studies have explored its presence in rodents in India. This study aimed to detect S. moniliformis in free-living bandicoots (Bandicota bengalensis and Bandicota indica) in Northeastern India and perform a phylogenetic analysis to assess its genetic relationship with global isolates.
Materials and methods: A total of 106 bandicoots (B. bengalensis, n = 76; B. indica, n = 30) were captured from various environments in Meghalaya, India. Fecal and tissue samples were collected and subjected to DNA extraction. Molecular detection of S. moniliformis was conducted using species-specific polymerase chain reaction (PCR) targeting the 16S ribosomal RNA gene. Positive amplicons were sequenced, analyzed using Basic Local Alignment Search Tool, and subjected to phylogenetic analysis.
Results: PCR-based detection revealed a fecal prevalence of 3.3% (1/30) in B. indica and 0% in B. bengalensis. No tissue samples tested positive for S. moniliformis. The detected isolate exhibited 100% sequence identity with previously reported S. moniliformis strains and 99.63% similarity to Streptobacillus notomytis. Phylogenetic analysis clustered the recovered isolate with human and rodent-derived S. moniliformis strains from multiple global regions, suggesting potential zoonotic transmission.
Conclusion: This study presents the first molecular detection of S. moniliformis in bandicoots from India, highlighting its zoonotic potential. Given its transmission risks through rodent bites and excreta contamination, public health surveillance is essential. Clinicians should consider RBF and HF in patients presenting with prolonged fever, particularly in rodent-endemic areas.
期刊介绍：
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