Juan Carlos Andreu-Ballester, Carolina Hurtado-Marcos, Carlos García-Ballesteros, Jaime Pérez-Griera, Fernando Izquierdo, Dolores Ollero, Ana Jiménez, Rafael Gil-Borrás, Antonio Llombart-Cussac, Francisca López-Chuliá, Carmen Cuéllar
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引用次数: 0
摘要
背景：目的：分析白细胞介素 7（IL-7）及其受体在 CD 患者组织中的基因表达：我们对 80 名 CD 患者的外周血进行了研究，并将他们与 80 名健康人进行了比较。研究了外周血中 αβ 和 γδ T 细胞的数量和凋亡情况，以及 CD 患者（n = 25）肠道组织中 αβ 和 γδ T 细胞的比例。通过定量 PCR 分析了组织中 IL-7、IL-2 受体亚基 γ [分化簇 132 (CD132)]、受体 α (CD127) 和 Caspase-3 的基因和蛋白表达。同时还分析了血清中的 IL-7 水平：结果：在 CD 患者中，观察到外周血中 γδ T 细胞数量减少，CD56+ αβ 和 γδ T 细胞凋亡增加（分别为 P < 0.0001 和 P < 0.01），T 亚群与其凋亡呈反相关。此外，这些患者组织中的 IL-7 基因表达和 IL-7 蛋白均有所增加。与对照组相比，组织中的 Caspase-3 滴度较低（P > 0.01）。组织中 CD8+γδ T 细胞比例下降（P < 0.01），并与外周血中的 IL-7 水平直接相关。CD患者组织中IL-2受体亚基γ（CD132）的表达大大降低（P＜0.05）：CD患者组织中IL-2受体亚基γ（CD132）基因表达量降低与γδT细胞免疫缺陷之间可能存在因果关系。
Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn's disease.
Background: A deficiency of γδ T cells has been described in Crohn's disease (CD).
Aim: To analyze the gene expression of interleukin 7 (IL-7) and its receptors in the tissues of patients with CD.
Methods: We studied the peripheral blood of 80 patients with CD, comparing them with a group of 80 healthy subjects. The number and apoptosis of αβ and γδ T cells in peripheral blood and the proportion of αβ and γδ T cells in the intestinal tissues of patients with CD (n = 25) were studied. The gene and protein expression of IL-7, IL-2 receptor subunit γ [cluster of differentiation 132 (CD132)], receptor α (CD127), and caspase-3 in tissues was analyzed by quantitative PCR. Serum IL-7 levels were also analyzed.
Results: In patients with CD, a decreased number of γδ T cells and an increase in the apoptosis of CD56+ αβ and γδ T cells in peripheral blood was observed (P < 0.0001 and P < 0.01) respectively, and there was an inverse correlation among T subsets and their apoptosis. In addition, IL-7 gene expression and IL-7 protein in the tissues of these patients were increased. The titers of caspase-3 in tissues were low vs control group (P > 0.01). The percentage of CD8+γδ T cells decreased in tissues (P < 0.01), and was directly related to IL-7 levels in peripheral blood. The expression of IL-2 receptor subunit γ (CD132) was greatly decreased in the tissues of patients with CD (P < 0.05).
Conclusion: There may be a cause-effect relationship between the lower gene expression of the IL-2 receptor subunit γ (CD132) in tissues of patients with CD and γδ T cells immunodeficiency.
期刊介绍：
The primary aims of the WJG are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in gastroenterology and hepatology.
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