无患子新多糖的化学结构解析和免疫调节活性

IF 2.1 4区医学 Q3 CHEMISTRY, MEDICINAL

Planta medica Pub Date : 2025-05-08 DOI:10.1055/a-2573-7718

Yifang Cui, Haitao Fan, Meng Sun, Xinyang He, Jie Li, Guangzhong Tu, Yanyan Jiang, Bin Liu

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引用次数: 0

摘要

研究了从无患子（SR）中分离出的一种新型均相多糖 SP4002501 的化学结构和免疫调节活性。采用 DEAE-纤维素和 Sepharose CL-6B 柱层析法纯化了 SP4002501。经鉴定，单糖成分为鼠李糖（Rha）、半乳糖醛酸（GalA）、半乳糖（Gal）和阿拉伯糖（Ara），摩尔比为 3.7：86.6：2.7：7.1。根据甲基化分析、部分水解、傅立叶变换红外光谱和核磁共振分析，SP4002501 以聚半乳糖醛酸单元为骨架，含有少量半乳糖（Gal）。侧链与半乳糖（Gal）的 C-3 连接，由鼠李糖（Rha）、半乳糖醛酸（GalA）、半乳糖（Gal）和阿拉伯糖（Ara）组成，其中阿拉伯糖（Ara）为末端糖。生物活性评估表明，SP4002501 可通过促进巨噬细胞增殖和吞噬作用来发挥免疫调节活性。

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Chemical Structural Elucidation and Immunomodulatory Activity of a New Polysaccharide from Saposhnikoviae Radix.

The chemical structure and immunomodulatory activity of a new homogeneous polysaccharide, SP4002501, isolated from Saposhnikoviae Radix (SR), were investigated. Purification of SP4002501 was performed by DEAE-Cellulose and Sepharose CL-6B column chromatography. The monosaccharidic constituents were identified as rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara) with a molar ratio of 3.7: 86.6: 2.7: 7.1. According to the methylation analysis, partial hydrolysis, FT-IR, and NMR analysis, SP4002501 had a backbone of polygalacturonic acid units with a small amount of galactose (Gal). Side chains are connected to C-3 of galactose (Gal) and consist of rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara), with arabinose (Ara) as terminal sugar. Biological activity assessment suggests that SP4002501 exhibits immunomodulatory activity through promoting macrophage proliferation and phagocytosis.

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来源期刊

Planta medica 医学-药学

CiteScore

5.10

自引率

3.70%

发文量

101

审稿时长

1.8 months

期刊介绍： Planta Medica is one of the leading international journals in the field of natural products – including marine organisms, fungi as well as micro-organisms – and medicinal plants. Planta Medica accepts original research papers, reviews, minireviews and perspectives from researchers worldwide. The journal publishes 18 issues per year. The following areas of medicinal plants and natural product research are covered: -Biological and Pharmacological Activities -Natural Product Chemistry & Analytical Studies -Pharmacokinetic Investigations -Formulation and Delivery Systems of Natural Products. The journal explicitly encourages the submission of chemically characterized extracts.