Soren Seifi, Keegan M Leckie, Ingrid Giles, Taylor O'Brien, John O MacKenzie, Marco Todesco, Loren H Rieseberg, Gregory J Baute, Jose M Celedon
{"title":"大麻抗白粉病新位点（PM2）的定位与鉴定。","authors":"Soren Seifi, Keegan M Leckie, Ingrid Giles, Taylor O'Brien, John O MacKenzie, Marco Todesco, Loren H Rieseberg, Gregory J Baute, Jose M Celedon","doi":"10.3389/fpls.2025.1543229","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Breeding genetic resistance to economically important crop diseases is the most sustainable strategy for disease management and enhancing agricultural and horticultural productivity, particularly where the application of synthetic pesticides is prohibited. Powdery mildew disease, caused by the biotrophic fungal pathogen <i>Golovinomyces ambrosiae</i>, is one of the most prevalent threats to the cannabis and hemp industry worldwide.</p><p><strong>Methods: </strong>In this study, we used bulked-segregant analysis combined with high-throughput RNA sequencing (BSRSeq) to identify and map a novel single dominant resistance (R) locus (designated PM2), that strongly suppresses powdery mildew infection and sporulation in <i>Cannabis sativa</i>.</p><p><strong>Results and discussion: </strong>BSA mapped PM2 to chromosome 9. Histochemical analysis revealed that PM2-induced resistance is mediated by a highly localized hypersensitive response mainly in the epidermal cells of the host. Importantly, genetic markers capable of tracking PM2 resistance in breeding populations were developed using associated SNPs identified in this study. The ability to track PM2 will allow for successful introgression of PM resistance into elite cannabis cultivars and help move towards a more sustainable cannabis industry.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1543229"},"PeriodicalIF":4.1000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966446/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mapping and characterization of a novel powdery mildew resistance locus (PM2) in <i>Cannabis sativa</i> L.\",\"authors\":\"Soren Seifi, Keegan M Leckie, Ingrid Giles, Taylor O'Brien, John O MacKenzie, Marco Todesco, Loren H Rieseberg, Gregory J Baute, Jose M Celedon\",\"doi\":\"10.3389/fpls.2025.1543229\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Breeding genetic resistance to economically important crop diseases is the most sustainable strategy for disease management and enhancing agricultural and horticultural productivity, particularly where the application of synthetic pesticides is prohibited. Powdery mildew disease, caused by the biotrophic fungal pathogen <i>Golovinomyces ambrosiae</i>, is one of the most prevalent threats to the cannabis and hemp industry worldwide.</p><p><strong>Methods: </strong>In this study, we used bulked-segregant analysis combined with high-throughput RNA sequencing (BSRSeq) to identify and map a novel single dominant resistance (R) locus (designated PM2), that strongly suppresses powdery mildew infection and sporulation in <i>Cannabis sativa</i>.</p><p><strong>Results and discussion: </strong>BSA mapped PM2 to chromosome 9. Histochemical analysis revealed that PM2-induced resistance is mediated by a highly localized hypersensitive response mainly in the epidermal cells of the host. Importantly, genetic markers capable of tracking PM2 resistance in breeding populations were developed using associated SNPs identified in this study. The ability to track PM2 will allow for successful introgression of PM resistance into elite cannabis cultivars and help move towards a more sustainable cannabis industry.</p>\",\"PeriodicalId\":12632,\"journal\":{\"name\":\"Frontiers in Plant Science\",\"volume\":\"16 \",\"pages\":\"1543229\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966446/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Plant Science\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fpls.2025.1543229\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1543229","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Mapping and characterization of a novel powdery mildew resistance locus (PM2) in Cannabis sativa L.
Introduction: Breeding genetic resistance to economically important crop diseases is the most sustainable strategy for disease management and enhancing agricultural and horticultural productivity, particularly where the application of synthetic pesticides is prohibited. Powdery mildew disease, caused by the biotrophic fungal pathogen Golovinomyces ambrosiae, is one of the most prevalent threats to the cannabis and hemp industry worldwide.
Methods: In this study, we used bulked-segregant analysis combined with high-throughput RNA sequencing (BSRSeq) to identify and map a novel single dominant resistance (R) locus (designated PM2), that strongly suppresses powdery mildew infection and sporulation in Cannabis sativa.
Results and discussion: BSA mapped PM2 to chromosome 9. Histochemical analysis revealed that PM2-induced resistance is mediated by a highly localized hypersensitive response mainly in the epidermal cells of the host. Importantly, genetic markers capable of tracking PM2 resistance in breeding populations were developed using associated SNPs identified in this study. The ability to track PM2 will allow for successful introgression of PM resistance into elite cannabis cultivars and help move towards a more sustainable cannabis industry.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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