Esophageal Reconstruction with Myogenesis-Inducing Gene Transfected Mesenchymal Stem Cell-Seeded Film with Leaf-Stacked Structure.

In the domain of tissue engineering and regenerative medicine, artificial replacements have been developed as viable options for esophageal reconstruction and serve as alternatives to traditional surgical procedures. Restoration of smooth muscle functionality is crucial in esophageal regeneration. We evaluated the efficacy of esophageal reconstructions in an animal model, using tissue-engineered films with a leaf-stacked structure (FLSS), seeded with mesenchymal stem cells (MSCs), which were genetically modified with myogenic genes. Esophageal partial defects were variously reconstructed in animals (n = 8 per group, except the no-implantation group), categorized as (1) normal rats; (2) rats implanted with naked FLSS; (3) rats implanted with FLSS with MSCs; (4) rats implanted using FLSS with myogenesis-inducing gene transfected MSCs; and (5) rats without implantation at the defect site (n = 3). The FLSS exhibited appropriate mechanical characteristics for transplantation. Successful repair of esophageal defects was observed with significantly enhanced epithelial regeneration in the MSC-seeded FLSS group compared to that in the naked FLSS group. Moreover, smooth muscle regeneration was notably higher in the FLSS with myogenesis-inducing gene transfected MSCs than in the group without myogenic gene transfection. The myogenesis-inducing gene-transfected MSC-seeded FLSS group showed a tendency toward increased smooth muscle regeneration, this indicates that FLSS with myogenesis-inducing genes transfected MSC may contribute positively to the maintenance of function in the reconstructed esophagus.