减少周转时间的自动尿液培养系统:一个前瞻性的现实世界评估

IF 2.1 4区 医学 Q3 INFECTIOUS DISEASES
Lorena Porte , María Jesús Alfaro , Carmen Varela , Jorge Reyes , Thomas Weitzel
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引用次数: 0

摘要

背景:泌尿道感染是临床上最常见的细菌性疾病之一。尿培养是这些感染的诊断参考标准,占微生物实验室工作量的很大一部分。常规尿液培养需要18-24小时的孵育,80%为阴性。Uroquattro-HB&;L®[Alifax]是一种自动尿液培养系统,可提供5 h的结果。目的评估该快速系统与传统培养相比的诊断性能,并测试其在现实环境中快速识别和药敏试验的适用性。方法收集1062例尿样,采用uroquatro - hb&;L快速培养与常规培养并行检测。将uroquatro - hb&;L与常规培养物和复合参比标准物进行性能分析。用MALDI-TOF技术对快速培养的微生物进行鉴定。在58份uroquatro - hb&; l阳性样本中,使用VITEK 2 XL系统应用快速药敏试验方案。结果与常规培养相比,uroquatro - hb&;L的敏感性为87.3%,特异性为91.1%,准确性为90.5%,阳性预测值为64.9%,阴性预测值为97.4%。采用该标准品,灵敏度为91.3%,特异度为100%。鉴定结果的符合率为92.3%。结论suroquatro - hb&;L能准确识别培养阴性尿样,并能在4 ~ 5 h内识别阳性尿样,可获得快速药敏试验结果;然而,这个应用程序需要进一步的评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Automated urine culture system with reduced turnaround time: A prospective real-world evaluation

Background

Urinary tract infections are among the most common bacterial illnesses in clinical practice. Urine culture is the diagnostic reference standard for these infections, representing a major proportion of the workload of microbiological laboratories. Conventional urine cultures require 18-24 h of incubation and are negative in about 80.0 %. Uroquattro-HB&L® [Alifax] is an automated urine culture system providing results in 5 h.

Objectives

To evaluate the diagnostic performance of this rapid system compared to conventional culture and test its applicability for rapid identification and susceptibility testing in a real-world setting.

Methods

A total of 1,062 routine urine samples were included and tested in parallel by Uroquattro-HB&L rapid culture and by conventional culture. Uroquattro-HB&L performance was analyzed in comparison to conventional culture and a composite reference standard. Microorganisms from rapid culture were identified by MALDI-TOF technique. In a subset of 58 Uroquattro-HB&L-positive samples, a rapid susceptibility testing protocol was applied using the VITEK 2 XL system.

Results

Compared to conventional culture, Uroquattro-HB&L showed a sensitivity of 87.3 %, specificity of 91.1 %, accuracy of 90.5 %, and positive and negative predictive values of 64.9 % and 97.4 %, respectively. Using the composite reference standard, the sensitivity and specificity were 91.3 % and 100 %, respectively. Agreement between the identification results was 92.3 %. Rapid antimicrobial susceptibility testing showed >90.0 % concordance with conventional AST.

Conclusions

Uroquattro-HB&L accurately identified culture-negative urine samples and permitted the identification of positive samples within 4-5 h. The system was also used to obtain rapid susceptibility testing results; however, this application requires further evaluation.
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来源期刊
CiteScore
5.30
自引率
3.40%
发文量
149
审稿时长
56 days
期刊介绍: Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.
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