超秒跟踪和活细胞核型揭示有丝分裂染色体动力学原理

IF 17.3 1区 生物学 Q1 CELL BIOLOGY
Rumen Stamatov, Sonya Uzunova, Yoana Kicheva, Maria Karaboeva, Tavian Blagoev, Stoyno Stoynov
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引用次数: 0

摘要

有丝分裂染色体动力学对细胞周期中基因组的三维组织至关重要,但由于方法上的挑战,这一过程的时空特征仍不清楚。虽然Hi-C方法捕获染色体间接触,但它们缺乏单细胞时间动力学,而显微镜则与漂白和光毒性作斗争。在这里,为了克服这些限制,我们引入了有丝分裂管道中染色体的便利分割和跟踪(FAST CHIMP),将延时超分辨率显微镜与深度学习相结合。FAST黑猩猩以8秒的分辨率跟踪了从前期到末期的所有人类染色体,在单个细胞中鉴定了23对同源对中的15对,并比较了母细胞和子细胞之间的染色体定位。它揭示了一个中心体运动依赖流,控制着染色体在前期和中期板位置之间的映射。此外,FAST CHIMP测量了染色体内和染色体间接触的超秒动态。该工具为活细胞中染色质行为的研究增加了一个动态维度,有望超越现有方法的范围。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics

Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics

Mitotic chromosome dynamics are essential for the three-dimensional organization of the genome during the cell cycle, but the spatiotemporal characteristics of this process remain unclear due to methodological challenges. While Hi-C methods capture interchromosomal contacts, they lack single-cell temporal dynamics, whereas microscopy struggles with bleaching and phototoxicity. Here, to overcome these limitations, we introduce Facilitated Segmentation and Tracking of Chromosomes in Mitosis Pipeline (FAST CHIMP), pairing time-lapse super-resolution microscopy with deep learning. FAST CHIMP tracked all human chromosomes with 8-s resolution from prophase to telophase, identified 15 out of 23 homologue pairs in single cells and compared chromosomal positioning between mother and daughter cells. It revealed a centrosome-motion-dependent flow that governs the mapping between chromosome locations at prophase and their metaphase plate position. In addition, FAST CHIMP measured supra-second dynamics of intra- and interchromosomal contacts. This tool adds a dynamic dimension to the study of chromatin behaviour in live cells, promising advances beyond the scope of existing methods.

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来源期刊
Nature Cell Biology
Nature Cell Biology 生物-细胞生物学
CiteScore
28.40
自引率
0.90%
发文量
219
审稿时长
3 months
期刊介绍: Nature Cell Biology, a prestigious journal, upholds a commitment to publishing papers of the highest quality across all areas of cell biology, with a particular focus on elucidating mechanisms underlying fundamental cell biological processes. The journal's broad scope encompasses various areas of interest, including but not limited to: -Autophagy -Cancer biology -Cell adhesion and migration -Cell cycle and growth -Cell death -Chromatin and epigenetics -Cytoskeletal dynamics -Developmental biology -DNA replication and repair -Mechanisms of human disease -Mechanobiology -Membrane traffic and dynamics -Metabolism -Nuclear organization and dynamics -Organelle biology -Proteolysis and quality control -RNA biology -Signal transduction -Stem cell biology
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