IF 4.6 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Zubi Sadiq, Seyed Hamid Safiabadi Tali, Maryam Mansouri, Sana Jahanshahi-Anbuhi
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引用次数: 0

摘要

护理点(POC)设备提供现场疾病诊断,尤其是在资源有限的环境中。尽管 POC 检测取得了长足的进步,但商用设备的供应仍然有限,主要原因是检测灵敏度和便携性方面的挑战。此外,为了更好地满足最终用户的需求,现有 POC 设备的进步也是必不可少的。在此,我们介绍一种使用葡聚糖-金纳米颗粒(dAuNPs)的比色双功能片剂传感器,用于检测和量化尿液中的尿酸和葡萄糖水平。我们的片剂传感器结合了 dAuNPs 的质子和纳米酶特性,从而实现了对这两种生物标记物的高灵敏度检测。有趣的是,我们直接从 dAuNP 溶液中制造纳米金药片,无需添加任何外部稳定剂或成片试剂,因此将其命名为直接药片。由于葡聚糖和尿酸之间的氢键作用,我们采用了无酶方法检测尿酸,其检测范围为 0.00187-7.8 mM,检测限低至 0.0037 mM。另一方面,dAuNPs 具有独特的纳米酶特性,在葡萄糖检测方面具有独特的 POx 模仿活性(K m = 0.106 mM,V max = 369.72 mM min-1),检测限低至 0.625 mM。我们的双功能片剂在尿酸和葡萄糖的比色-色原检测中具有优异的底物选择性。这种双功能性不仅为资源有限的环境提供了一种高灵敏度、高选择性和高成本效益的检测策略,还为设计可定制的质子纳米酶纳米金片传感器作为快速诊断的有力工具开辟了一条新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A dual-functional nanogold tablet as a plasmonic and nanozyme sensor for point-of-care applications.

Point-of-care (POC) devices provide on-site disease diagnosis, particularly in resource-limited settings. Despite considerable progress in POC testing, the availability of commercial devices remains limited, primarily due to challenges in detection sensitivity and portability. Furthermore, advancements in existing POC devices are essential to better meet the needs of end-users. Herein, we present a colorimetric dual-functional tablet sensor using dextran-gold nanoparticles (dAuNPs) to detect and quantify uric acid and glucose levels in urine. Our tablet sensor combines the plasmonic and nanozyme properties of dAuNPs, resulting in highly sensitive detection of both biomarkers. Interestingly, we fabricated the nanogold tablet directly from the dAuNP solution without the addition of any external stabilizer or tablet-forming reagent, thus naming it a direct tablet. An enzyme-free approach was employed for uric acid detection, providing a wide detection range of 0.00187-7.8 mM and a low detection limit of 0.0037 mM, attributed to the hydrogen bonding between dextran and uric acid. On the other hand, the unique nanozyme properties of dAuNPs exhibited exclusive POx-mimetic activity for glucose detection (K m = 0.106 mM and V max = 369.72 mM min-1), with a lower detection limit of 0.625 mM. Our dual-functional tablet offers exceptional substrate selectivity for the colorimetric-chromogenic assay of both uric acid and glucose. This dual-functionality not only provides a highly sensitive, selective, and cost-effective detection strategy for resource-limited settings but also introduces a new avenue for designing customizable plasmonic-nanozyme nanogold tablet sensors as a powerful tool for rapid diagnosis.

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来源期刊
Nanoscale Advances
Nanoscale Advances Multiple-
CiteScore
8.00
自引率
2.10%
发文量
461
审稿时长
9 weeks
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