Samuel Kwasi Appiah, Charles Nkansah, Godfred Amoah Appiah, Gabriel Abbam, Felix Osei-Boakye, Samira Daud, Kofi Mensah, Safo Adwoa, Korah Seedolf Kuwaahsuore, Emmanuel Yeboah, Abu Siraj Salma Tiyumba, Dennis Thompson, Viel Mary Paula, Louis Adda Duibajia, Peter Takyia, Franklina Ataa Kwarteng, Obed Odame Asiedu, Firdaus Ibrahim Sukasorr, Vincent Kawuribi, Boniface Nwofoke Ukwah, Ejike Felix Chukwurah
{"title":"红细胞铁素驱动的镰状细胞贫血患者Hepcidin和铁水平调节：一项前瞻性研究。","authors":"Samuel Kwasi Appiah, Charles Nkansah, Godfred Amoah Appiah, Gabriel Abbam, Felix Osei-Boakye, Samira Daud, Kofi Mensah, Safo Adwoa, Korah Seedolf Kuwaahsuore, Emmanuel Yeboah, Abu Siraj Salma Tiyumba, Dennis Thompson, Viel Mary Paula, Louis Adda Duibajia, Peter Takyia, Franklina Ataa Kwarteng, Obed Odame Asiedu, Firdaus Ibrahim Sukasorr, Vincent Kawuribi, Boniface Nwofoke Ukwah, Ejike Felix Chukwurah","doi":"10.1155/bmri/6803051","DOIUrl":null,"url":null,"abstract":"<p><p>The interplay of erythroferrone (ERFE), hepcidin, and ferroportin is crucial for ensuring systemic iron homeostasis. This study determined the influence of ERFE on hepcidin and iron levels in polytransfused patients with sickle cell anaemia (SCA). This multicentre case-control study recruited 60 SCA participants and 30 controls (HbA), aged 2-34 years, from Tamale Teaching Hospital; Methodist Hospital, Wenchi; and Seventh Day Adventist Hospital, Sunyani, Ghana, between the periods of March to July 2023. About 4 mL of blood was collected for a full blood count using a haematology analyzer and serum ERFE, hepcidin, ferroportin, and ferritin estimation using an enzyme-linked immunosorbent assay. Data were analyzed using SPSS Version 26.0. ERFE (<i>p</i> < 0.001), ferroportin (<i>p</i> = 0.016), ferritin (<i>p</i> < 0.001), serum iron (<i>p</i> < 0.001), transferrin (<i>p</i> = 0.001), soluble transferrin receptor (sTFR) (<i>p</i> = 0.019), TWBC (<i>p</i> < 0.001), and platelet (<i>p</i> < 0.001) were significantly higher in SCA participants and hydroxyurea-naïve participants than in the control group and hydroxyurea-treated participants, respectively. Levels of hepcidin (<i>p</i> < 0.001), red blood cell (<i>p</i> < 0.001), haemoglobin (<i>p</i> < 0.001), and haematocrit (<i>p</i> < 0.001) were lower in the SCA and hydroxyurea-naïve groups than in the control and hydroxyurea-treated groups, respectively. An inverse correlation was observed between serum ERFE and hepcidin (<i>r</i> = -0.391, <i>p</i> = 0.002) and hepcidin and ferroportin (<i>r</i> = -0.266, <i>p</i> = 0.040), while ferritin (<i>r</i> = 0.439, <i>p</i> < 0.001) and ferroportin (<i>r</i> = 0.309, <i>p</i> = 0.016) showed a positive correlation with ERFE. No correlation was found between serum hepcidin and ferritin levels (<i>r</i> = 0.025, <i>p</i> = 0.853). Again, participants with regular blood transfusions had significantly higher levels of ERFE (<i>p</i> < 0.001) and ferritin (<i>p</i> = 0.002) than those with rare and no transfusions per year. None of the SCA participants had done iron testing. In conclusion, the negative impact of ERFE on hepcidin levels may exacerbate the risk of iron burden, as evident by elevated iron levels in SCA patients and the need for regular monitoring of the iron status of polytransfused SCA patients.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6803051"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964725/pdf/","citationCount":"0","resultStr":"{\"title\":\"Erythroferrone-Driven Regulation of Hepcidin and Iron Levels in Polytransfused Sickle Cell Anaemia Patients: A Prospective Study.\",\"authors\":\"Samuel Kwasi Appiah, Charles Nkansah, Godfred Amoah Appiah, Gabriel Abbam, Felix Osei-Boakye, Samira Daud, Kofi Mensah, Safo Adwoa, Korah Seedolf Kuwaahsuore, Emmanuel Yeboah, Abu Siraj Salma Tiyumba, Dennis Thompson, Viel Mary Paula, Louis Adda Duibajia, Peter Takyia, Franklina Ataa Kwarteng, Obed Odame Asiedu, Firdaus Ibrahim Sukasorr, Vincent Kawuribi, Boniface Nwofoke Ukwah, Ejike Felix Chukwurah\",\"doi\":\"10.1155/bmri/6803051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The interplay of erythroferrone (ERFE), hepcidin, and ferroportin is crucial for ensuring systemic iron homeostasis. This study determined the influence of ERFE on hepcidin and iron levels in polytransfused patients with sickle cell anaemia (SCA). This multicentre case-control study recruited 60 SCA participants and 30 controls (HbA), aged 2-34 years, from Tamale Teaching Hospital; Methodist Hospital, Wenchi; and Seventh Day Adventist Hospital, Sunyani, Ghana, between the periods of March to July 2023. About 4 mL of blood was collected for a full blood count using a haematology analyzer and serum ERFE, hepcidin, ferroportin, and ferritin estimation using an enzyme-linked immunosorbent assay. Data were analyzed using SPSS Version 26.0. ERFE (<i>p</i> < 0.001), ferroportin (<i>p</i> = 0.016), ferritin (<i>p</i> < 0.001), serum iron (<i>p</i> < 0.001), transferrin (<i>p</i> = 0.001), soluble transferrin receptor (sTFR) (<i>p</i> = 0.019), TWBC (<i>p</i> < 0.001), and platelet (<i>p</i> < 0.001) were significantly higher in SCA participants and hydroxyurea-naïve participants than in the control group and hydroxyurea-treated participants, respectively. Levels of hepcidin (<i>p</i> < 0.001), red blood cell (<i>p</i> < 0.001), haemoglobin (<i>p</i> < 0.001), and haematocrit (<i>p</i> < 0.001) were lower in the SCA and hydroxyurea-naïve groups than in the control and hydroxyurea-treated groups, respectively. An inverse correlation was observed between serum ERFE and hepcidin (<i>r</i> = -0.391, <i>p</i> = 0.002) and hepcidin and ferroportin (<i>r</i> = -0.266, <i>p</i> = 0.040), while ferritin (<i>r</i> = 0.439, <i>p</i> < 0.001) and ferroportin (<i>r</i> = 0.309, <i>p</i> = 0.016) showed a positive correlation with ERFE. No correlation was found between serum hepcidin and ferritin levels (<i>r</i> = 0.025, <i>p</i> = 0.853). Again, participants with regular blood transfusions had significantly higher levels of ERFE (<i>p</i> < 0.001) and ferritin (<i>p</i> = 0.002) than those with rare and no transfusions per year. None of the SCA participants had done iron testing. In conclusion, the negative impact of ERFE on hepcidin levels may exacerbate the risk of iron burden, as evident by elevated iron levels in SCA patients and the need for regular monitoring of the iron status of polytransfused SCA patients.</p>\",\"PeriodicalId\":9007,\"journal\":{\"name\":\"BioMed Research International\",\"volume\":\"2025 \",\"pages\":\"6803051\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964725/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BioMed Research International\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1155/bmri/6803051\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioMed Research International","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/bmri/6803051","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Erythroferrone-Driven Regulation of Hepcidin and Iron Levels in Polytransfused Sickle Cell Anaemia Patients: A Prospective Study.
The interplay of erythroferrone (ERFE), hepcidin, and ferroportin is crucial for ensuring systemic iron homeostasis. This study determined the influence of ERFE on hepcidin and iron levels in polytransfused patients with sickle cell anaemia (SCA). This multicentre case-control study recruited 60 SCA participants and 30 controls (HbA), aged 2-34 years, from Tamale Teaching Hospital; Methodist Hospital, Wenchi; and Seventh Day Adventist Hospital, Sunyani, Ghana, between the periods of March to July 2023. About 4 mL of blood was collected for a full blood count using a haematology analyzer and serum ERFE, hepcidin, ferroportin, and ferritin estimation using an enzyme-linked immunosorbent assay. Data were analyzed using SPSS Version 26.0. ERFE (p < 0.001), ferroportin (p = 0.016), ferritin (p < 0.001), serum iron (p < 0.001), transferrin (p = 0.001), soluble transferrin receptor (sTFR) (p = 0.019), TWBC (p < 0.001), and platelet (p < 0.001) were significantly higher in SCA participants and hydroxyurea-naïve participants than in the control group and hydroxyurea-treated participants, respectively. Levels of hepcidin (p < 0.001), red blood cell (p < 0.001), haemoglobin (p < 0.001), and haematocrit (p < 0.001) were lower in the SCA and hydroxyurea-naïve groups than in the control and hydroxyurea-treated groups, respectively. An inverse correlation was observed between serum ERFE and hepcidin (r = -0.391, p = 0.002) and hepcidin and ferroportin (r = -0.266, p = 0.040), while ferritin (r = 0.439, p < 0.001) and ferroportin (r = 0.309, p = 0.016) showed a positive correlation with ERFE. No correlation was found between serum hepcidin and ferritin levels (r = 0.025, p = 0.853). Again, participants with regular blood transfusions had significantly higher levels of ERFE (p < 0.001) and ferritin (p = 0.002) than those with rare and no transfusions per year. None of the SCA participants had done iron testing. In conclusion, the negative impact of ERFE on hepcidin levels may exacerbate the risk of iron burden, as evident by elevated iron levels in SCA patients and the need for regular monitoring of the iron status of polytransfused SCA patients.
期刊介绍：
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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