推进脑类器官电生理:综合表征的微创技术

IF 6.4 3区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Mujeeb Yousuf, Jean-Christophe (Chris) Rochet, Pushpapraj Singh, Muhammad Mustafa Hussain
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引用次数: 0

摘要

人类大脑类器官起源于多能干细胞,是广泛研究工作的宝贵工具,可以复制大脑功能。它们复制细胞相互作用、形态和分裂的能力为大脑发育、疾病建模和药物筛选提供了宝贵的见解。然而,传统的形态学分析方法往往是侵入性的,缺乏实时监测能力,对实现全面的理解构成限制。因此,推进对脑类器官电生理的理解需要开发具有长期、高分辨率能力的微创测量技术。本文综述了人脑类器官的研究意义,强调了电生理表征的必要性。它深入研究了传统的评估方法,特别关注3D微电极阵列,电极插入机制,以及柔性电极阵列促进微创记录的重要性。此外,还介绍了用于监测类器官特性的各种传感器,丰富了对其化学,热和机械动力学的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Advancing Brain Organoid Electrophysiology: Minimally Invasive Technologies for Comprehensive Characterization

Advancing Brain Organoid Electrophysiology: Minimally Invasive Technologies for Comprehensive Characterization

Human brain organoids, which originate from pluripotent stem cells, serve as valuable tools for a wide range of research endeavors, replicating brain function. Their capacity to replicate cellular interactions, morphology, and division provides invaluable insights into brain development, disease modeling, and drug screening. However, conventional morphological analysis methods are often invasive and lack real-time monitoring capabilities, posing limitations to achieving a comprehensive understanding. Therefore, advancing the comprehension of brain organoid electrophysiology necessitates the development of minimally invasive measurement technologies with long-term, high-resolution capabilities. This review highlights the significance of human brain organoids and emphasizes the need for electrophysiological characterization. It delves into conventional assessment methods, particularly focusing on 3D microelectrode arrays, electrode insertion mechanisms, and the importance of flexible electrode arrays to facilitate minimally invasive recordings. Additionally, various sensors tailored to monitor organoid properties are introduced, enriching the understanding of their chemical, thermal, and mechanical dynamics.

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来源期刊
Advanced Materials Technologies
Advanced Materials Technologies Materials Science-General Materials Science
CiteScore
10.20
自引率
4.40%
发文量
566
期刊介绍: Advanced Materials Technologies Advanced Materials Technologies is the new home for all technology-related materials applications research, with particular focus on advanced device design, fabrication and integration, as well as new technologies based on novel materials. It bridges the gap between fundamental laboratory research and industry.
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