DLX3/SAHH轴对牙槽骨骨髓间充质干细胞成骨分化的影响

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences Pub Date : 2025-04-01 DOI:10.1016/j.job.2025.100658

Shichen Sun , Yuxi Jiang , Kang Chen , Xinyi Chen , Jinyou Chai , Haipeng Sun

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引用次数: 0

摘要

目的骨髓间充质干细胞（BMSCs）促进牙槽骨的形成和修复。远端无同源盒3 （DLX3）在BMSC成骨过程中起关键调节作用。然而，它介导骨髓间充质干细胞成骨的确切途径尚不清楚。在这项研究中，我们研究了dlx3介导的骨髓间充质干细胞成骨的潜在表观遗传机制。方法从牙槽骨中分离骨髓间充质干细胞。通过慢病毒介导的基因转移，建立了DLX3过表达和低表达细胞系。采用碱性磷酸酶表达、茜素红染色、实时定量聚合酶链反应、western blotting、染色质免疫沉淀、RNA免疫沉淀和s -腺苷-同型半胱氨酸水解酶（SAHH）活性测定来评估成骨分化。结果dlx3促进骨髓间充质干细胞成骨分化，正向调节SAHH，增强zeste同源物2 （EZH2）活性。此外，在骨髓间充质干细胞中，长链非编码RNA H19 （H19）与SAHH结合。DLX3调节H19的表达，救援实验表明，H19敲低可提高SAHH活性，从而促进DLX3过表达组的成骨分化。结论DLX3/SAHH轴可能调控EZH2的活性，参与骨髓间充质干细胞成骨分化。

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Impact of DLX3/SAHH axis on osteogenic differentiation of BMSCs in alveolar bone

Objectives

Bone marrow mesenchymal stem cells (BMSCs) promote alveolar bone formation and repair. Distal-less homeobox 3 (DLX3) plays a key regulatory role in BMSC osteogenesis. However, the precise pathway by which it mediates osteogenesis in BMSCs remains unclear. In this study, we investigated the potential epigenetic mechanisms underlying DLX3-mediated BMSCs osteogenesis.

Methods

BMSCs were isolated from the alveolar bone. DLX3 overexpression and knockdown cell lines were established using lentivirus-mediated gene transfer. Osteogenic differentiation was evaluated using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction, western blotting, chromatin immunoprecipitation, RNA immunoprecipitation, and S-adenosyl-homocysteine hydrolase (SAHH) activity measurements.

Results

DLX3 enhanced the osteogenic differentiation of BMSCs, positively regulated SAHH, and enhancer of zeste homolog 2 (EZH2) activity. In addition, the long non-coding RNA H19 (H19) bound to SAHH in BMSCs. DLX3 regulated the expression of H19, and rescue experiments showed that H19 knockdown increased SAHH activity, thereby promoting osteogenic differentiation in the DLX3-overexpression group.