用亲水性修饰的叶绿素衍生CDs实现深红色的水致发光成像应用

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-04-01 DOI:10.1016/j.dyepig.2025.112799

Jiahui Zhang , Wenjing Huang , Lie Li , Linyun Zeng , Chunli Li , Qiyu Zhan , Ruiyuan Liu , Jialiang Pan , Jinqing Huang , Wei Liu , Xinguo Zhang

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引用次数: 0

摘要

叶绿素衍生的碳点（CDs）具有强烈的深红色发射，是近红外窗口中高质量生物成像的理想候选者。但其在水中分散性低，发射猝灭严重，限制了其生物应用。本研究通过一种简单温和的溶液混合方法，在室温下成功地将桑叶叶绿素衍生CDs （mcd）涂覆在聚乙烯亚胺（PEI）上。实验优化证明，反应温度为120℃，反应时间为4 h时制备的mcd具有最佳的PL强度，而PEI/CDs比为0.4‰时制备的PEI- mcd具有最佳的PL强度。PEI-MCDs复合材料在紫外线照射、温度波动、盐和各种物质等条件下均表现出良好的水性分散性和优异的荧光稳定性。在PEI-MCDs水溶液中获得了670 nm的强窄带深红色发射，量子产率为45.44%。在水溶液中亲脂-亲水转化和发射回收的机理证实是PEI在mcd上的两亲性壳层，有效地提高了水溶液的分散性，抑制了聚集猝灭的发生。利用pei - mcd实现了各种细胞中溶酶体的选择性成像，而不靶向配体。此外，斑马鱼胚胎和幼虫的体内荧光成像显示出清晰的组织结构。结果表明，pei - mcd具有显著的两亲性、优异的稳定性和优异的深红色发射特性，在生物成像领域具有广阔的应用前景。

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Realization of bright deep-red aqueous luminescence from chlorophyll-derived CDs with hydrophilic modification for imaging application

Chlorophyll-derived carbon dots (CDs) with strong deep red emission is an ideal candidate for high-quality bio-imaging in the NIR window. However, its low aqueous dispersity with severe emission quenching has limited its biological application. Herein, chlorophyll-derived CDs from mulberry leaf (MCDs) have been successfully coated with polyethylenimine (PEI) via a simple and mild solution-mixing method at room temperature. Experimental optimization proved that the MCDs produced at a reaction temperature of 120 °C and a reaction time of 4 h had the best PL strength, while the PEI-MCDs produced at a PEI/CDs ratio of 0.4 ‰ had the best PL strength. The PEI-MCDs composites demonstrate good aqueous dispersity and exceptional fluorescence stability under various conditions like UV irradiation, temperature fluctuations, salt and various substances. A strong narrow-band deep-red emission at 670 nm with quantum yield (QY) of 45.44 % is obtained in PEI-MCDs aqueous solution. The mechanism of lipophilic-hydrophilic transformation and emission recovery in aqueous solution is confirmed to be the amphiphilicity shell of PEI on MCDs, which effectively improves the aqueous dispersity and suppresses the occurrence of aggregation-caused quenching. Selective imaging of lysosomes in various cells has been realized by utilized PEI-MCDs without targeting ligands. Furthermore, clear tissue structures were shown in the in vivo fluorescence imaging of zebrafish embryos and larvae. The results indicate PEI-MCDs own notable amphiphilicity, superior stability and excellent deep-red emission, which hold great promise for biological imaging applications.

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.