槲皮素对模型蛋白牛血清白蛋白的稳定作用

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-28 DOI:10.1016/j.colsurfb.2025.114663

Amit G. Rathod , Priyanka Tiwari , Jatin Shaily, Sanjay Tiwari

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引用次数: 0

摘要

槲皮素（QCT）是一类新兴的类黄酮，具有抗氧化和抗炎活性，其蛋白质稳定作用已被研究。在证明乙醇（EtOH）诱导牛血清白蛋白（BSA）结构变化后，利用荧光、圆二色（CD）和傅里叶变换红外（FTIR）光谱技术研究了QCT的稳定作用。用原子力显微镜（AFM）观察形态学变化。乙醇浓度越高，牛血清白蛋白荧光光谱蓝移越明显。在QCT的存在下，这一趋势发生了逆转。这被解释为抗淀粉样蛋白的作用是由QCT与牛血清白蛋白的疏水口袋结合而产生的。结合常数(1.25 × 106 M−1；298 K)提示QCT对BSA有很强的结合亲和力。发现qct诱导的荧光猝灭模式具有混合性。CD谱显示，EtOH的存在改变了蛋白质的构象，α螺旋的痕迹消失。相反，加入QCT后，EtOH的破坏效应不明显。热CD数据进一步证实了这一点，表明BSA的变性温度升高。硫黄酮T试验和AFM实验数据进一步证实了QCT的保护作用。

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Stabilizing effect of quercetin upon bovine serum albumin as a model protein

Quercetin (QCT), an emerging class of flavonoid known for antioxidant and anti-inflammatory activities, has been studied for its protein stabilizing effect. After demonstrating ethanol (EtOH) - induced structural changes in bovine serum albumin (BSA), the stabilizing effect of QCT was studied using fluorescence, circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopic techniques. Morphological changes were examined using atomic force microscopy (AFM). EtOH triggered blue shift in fluorescence spectra of BSA and its intensity increased at higher percentage of alcohol. A reversal in this trend was recorded in the presence of QCT. This was interpreted as anti-amyloidogenic effect emanating from the binding of QCT to hydrophobic pockets of BSA. The value of binding constant (1.25 x 10⁶ M⁻¹; 298 K) is suggestive of strong binding affinity of QCT for BSA. The mode of QCT-induced fluorescence quenching was found to be mixed in nature. CD spectra showed that the protein conformation was altered and traces of alpha helix disappeared in the presence of EtOH. Contrarily, disruptive effect of EtOH was not visible upon incorporating QCT. This was further verifiable form the thermal CD data, which showed an upshift in the denaturation temperature of BSA. The data of thioflavin T assay and AFM further substantiated the protective effect of QCT.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.