使用和不使用纳米银的胆道塑料支架的比较:生物膜形成的体外研究。

Acta cirurgica brasileira Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI:10.1590/acb402825
Victor Kalil Flumignan, Marcelo de Palma Sircili, Marcia Regina Franzolin, Ana Marisa Chudzinski Tavassi, Lígia Garcia Germano, Ana Vitória Dos Santos Souza, Nicole Fernandes Silva, Newton Kiyoshi Fukumasu, Raphaela Marques Dos Anjos, Jose Pinhata Otoch, Everson Luiz de Almeida Artifon
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引用次数: 0

摘要

目的:塑料胆道支架是治疗胆道梗阻的一种经济有效的方法。不幸的是,它们的通畅度较低,这与腔内生物膜的形成有关。银纳米颗粒因其抗菌性能在生物医学领域的应用日益广泛。本研究旨在比较有银纳米颗粒涂层和没有银纳米颗粒涂层的支架与不同细菌培养基接触时生物膜的形成情况。方法:对不同类型的银膜在塑料胆道支架上的应用进行了试验。对两组支架进行分析:一组有不同类型的纳米银涂层,另一组没有涂层。将支架放置在不同的细菌培养基中并评估生物膜的形成。使用共聚焦显微镜和直接菌落形成单位(CFU/cm2)进行分析。结果:定量分析显示,C16包被组的大肠杆菌ATCC和铜绿假单胞菌ATCC在agnp包被组的生长速度下降(p < 0.05)。然而,当测试混合样品时,包括临床菌株和金黄色葡萄球菌,AgNP涂层没有抑制细菌的生长。结论:agnp包被支架对大肠杆菌ATCC和铜绿假单胞菌均有较好的抑制作用。需要进一步的研究来探索涂层机理的潜在改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison between biliary plastic stents with and without application of silver nanoparticles: an in-vitro study of the biofilm formation.

Purpose: Plastic biliary stents are a cost-effective treatment for biliary obstruction. Unfortunately, they have low patency, related to intraluminal biofilm formation. Silver nanoparticles (AgNPs) have been increasingly used in biomedicine because of its antibacterial properties. This study aimed to compare biofilm formation on stents with and without silver nanoparticle coatings when in contact with different bacterial culture medium.

Methods: Different types of silver coatings were tested on plastic biliary stents. Two groups of stents were analyzed: one group with various types of silver nanoparticle coatings, and a negative control group with no coating. The stents were placed in different bacterial culture media and assessed for biofilm formation. Analysis was performed using confocal microscopy and direct colony-forming unit (CFU/cm2).

Results: Quantitative analysis showed promising results with C16 coating, as Escherichia coli ATCC and Pseudomonas aeruginosa ATCC exhibited reduced growth in the AgNP-coated group (p < 0.05). However, when mixed samples, including clinical strains and Staphylococcus aureus, were tested, the AgNP coating did not inhibit bacterial growth.

Conclusion: AgNP-coated stents are effective against certain strains, such as E. coli ATCC and P. aeruginosa. Further research is needed to explore potential improvements in the coating mechanism.

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