MiR-93 promotes the progression and chemoresistance of diffuse large B-cell lymphoma cells via targeting SMAD5.

ABSTARCTTo investigate the role and mechanism of miR-93 in apoptosis, migration, invasion and chemoresistance of diffuse large B-cell lymphoma (DLBCL) cells, bioinformatics and real-time quantitative reverse transcription PCR were employed to detect the expression of miR-93 and SMAD5 in DLBCL tissues and cells. There was a notable upregulation of miR-93 expression in DLBCL samples. In DLBCL cells (OCI-LY7 and SU-DHL-8), miR-93 enhanced the chemoresistance to vincristine and inhibited apoptosis by increasing the expression of the anti-apoptotic protein Bcl-2 and decreasing cleaved caspase-3 levels. Additionally, miR-93 significantly reduced apoptosis rates and promoted cell migration and invasion. Collectively, miR-93 promoted malignant behaviour and increased chemoresistance of DLBCL cells by targeting and inhibiting SMAD5 expression. Thus, inhibiting miR-93 expression or elevating SMAD5 expression is likely to be crucial for DLBCL treatment.