{"title":"MiR-93通过靶向SMAD5促进弥漫性大b细胞淋巴瘤的进展和化疗耐药。","authors":"Xiang Fang, Qiang Huang, Li-Jun Dong, Qi-Huan Liu, Xiao-Na Miao, Jian Xiong, Xiao-Duan Jiang, Qiang Yu","doi":"10.1080/13813455.2024.2404099","DOIUrl":null,"url":null,"abstract":"<p><p>ABSTARCTTo investigate the role and mechanism of miR-93 in apoptosis, migration, invasion and chemoresistance of diffuse large B-cell lymphoma (DLBCL) cells, bioinformatics and real-time quantitative reverse transcription PCR were employed to detect the expression of miR-93 and SMAD5 in DLBCL tissues and cells. There was a notable upregulation of miR-93 expression in DLBCL samples. In DLBCL cells (OCI-LY7 and SU-DHL-8), miR-93 enhanced the chemoresistance to vincristine and inhibited apoptosis by increasing the expression of the anti-apoptotic protein Bcl-2 and decreasing cleaved caspase-3 levels. Additionally, miR-93 significantly reduced apoptosis rates and promoted cell migration and invasion. Collectively, miR-93 promoted malignant behaviour and increased chemoresistance of DLBCL cells by targeting and inhibiting SMAD5 expression. Thus, inhibiting miR-93 expression or elevating SMAD5 expression is likely to be crucial for DLBCL treatment.</p>","PeriodicalId":8331,"journal":{"name":"Archives of Physiology and Biochemistry","volume":" ","pages":"410-418"},"PeriodicalIF":2.5000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"MiR-93 promotes the progression and chemoresistance of diffuse large B-cell lymphoma cells via targeting SMAD5.\",\"authors\":\"Xiang Fang, Qiang Huang, Li-Jun Dong, Qi-Huan Liu, Xiao-Na Miao, Jian Xiong, Xiao-Duan Jiang, Qiang Yu\",\"doi\":\"10.1080/13813455.2024.2404099\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>ABSTARCTTo investigate the role and mechanism of miR-93 in apoptosis, migration, invasion and chemoresistance of diffuse large B-cell lymphoma (DLBCL) cells, bioinformatics and real-time quantitative reverse transcription PCR were employed to detect the expression of miR-93 and SMAD5 in DLBCL tissues and cells. There was a notable upregulation of miR-93 expression in DLBCL samples. In DLBCL cells (OCI-LY7 and SU-DHL-8), miR-93 enhanced the chemoresistance to vincristine and inhibited apoptosis by increasing the expression of the anti-apoptotic protein Bcl-2 and decreasing cleaved caspase-3 levels. Additionally, miR-93 significantly reduced apoptosis rates and promoted cell migration and invasion. Collectively, miR-93 promoted malignant behaviour and increased chemoresistance of DLBCL cells by targeting and inhibiting SMAD5 expression. Thus, inhibiting miR-93 expression or elevating SMAD5 expression is likely to be crucial for DLBCL treatment.</p>\",\"PeriodicalId\":8331,\"journal\":{\"name\":\"Archives of Physiology and Biochemistry\",\"volume\":\" \",\"pages\":\"410-418\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Physiology and Biochemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/13813455.2024.2404099\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Physiology and Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/13813455.2024.2404099","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/1 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
MiR-93 promotes the progression and chemoresistance of diffuse large B-cell lymphoma cells via targeting SMAD5.
ABSTARCTTo investigate the role and mechanism of miR-93 in apoptosis, migration, invasion and chemoresistance of diffuse large B-cell lymphoma (DLBCL) cells, bioinformatics and real-time quantitative reverse transcription PCR were employed to detect the expression of miR-93 and SMAD5 in DLBCL tissues and cells. There was a notable upregulation of miR-93 expression in DLBCL samples. In DLBCL cells (OCI-LY7 and SU-DHL-8), miR-93 enhanced the chemoresistance to vincristine and inhibited apoptosis by increasing the expression of the anti-apoptotic protein Bcl-2 and decreasing cleaved caspase-3 levels. Additionally, miR-93 significantly reduced apoptosis rates and promoted cell migration and invasion. Collectively, miR-93 promoted malignant behaviour and increased chemoresistance of DLBCL cells by targeting and inhibiting SMAD5 expression. Thus, inhibiting miR-93 expression or elevating SMAD5 expression is likely to be crucial for DLBCL treatment.
期刊介绍:
Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders.
The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications.
Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics:
-Dysregulation of hormone receptors and signal transduction
-Contribution of gene variants and gene regulatory processes
-Impairment of intermediary metabolism at the cellular level
-Secretion and metabolism of peptides and other factors that mediate cellular crosstalk
-Therapeutic strategies for managing metabolic diseases
Special issues dedicated to topics in the field will be published regularly.