将 CMOS 微型传感器无缝集成到开放式微流体系统中。

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-04-02 DOI:10.1039/d4lc01000k
Raziyeh Bounik, Alex E Landolt, Jihyun Lee, Vijay Viswam, Fernando Cardes, Mario M Modena, Andreas Hierlemann
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引用次数: 0

摘要

由于传统的二维(2D)细胞培养对药物开发的预测能力有限,三维(3D)组织模型,如球形微组织,已经被引入来更好地再现生理条件。在气液界面培养微组织的悬垂滴法对微组织的形成和维持是有效的。使用该技术,可以将承载不同人体器官模型的几个悬挂液滴流体互连,以概括相关组织的相互作用。在这里,我们将微流控与微电子(即互补金属氧化物半导体(CMOS)技术)相结合,并提出了一种集成到开放式微流控系统中的新型多功能CMOS微电极阵列(MEA)。该装置可以在悬挂-滴模式下用于原位显微组织读数,也可以像传统的MEA一样在立滴模式下使用。CMOS-MEA芯片具有两个可重构电极阵列,每个电极有1024个电极,并使电生理学,阻抗光谱和电化学传感能够获得广泛的生物相关信息。我们采用0.18 μm CMOS工艺制作芯片,并制定了将CMOS- mea芯片集成到开放式微流控系统中的策略,从而将离散CMOS传感器集成到微流控器件中。概念验证实验证明了对人类诱导多能干细胞(hiPSC)衍生的心脏微组织进行电生理学和阻抗谱分析的能力,以及对包括过氧化氢和肾上腺素在内的不同分析物进行电化学传感的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Seamless integration of CMOS microsensors into open microfluidic systems.

As traditional two-dimensional (2D) cell cultures offer limited predictive capabilities for drug development, three-dimensional (3D) tissue models, such as spherical microtissues, have been introduced to better reproduce physiological conditions. The hanging-drop method, used to cultivate microtissues at an air-liquid interface, proves to be effective for microtissue formation and maintenance. Using that technology, it is possible to fluidically interconnect several hanging drops hosting different models of human organs to recapitulate relevant tissue interactions. Here, we combine microfluidics with microelectronics (i.e., complementary metal-oxide-semiconductor (CMOS) technology) and present a novel multifunctional CMOS microelectrode array (MEA) integrated into an open microfluidic system. The device can be used in hanging-drop mode for in situ microtissue readouts and in standing-drop mode like a conventional MEA. The CMOS-MEA chip features two reconfigurable electrode arrays with 1024 electrodes each, and enables electrophysiology, impedance spectroscopy, and electrochemical sensing to acquire a broad spectrum of biologically relevant information. We fabricated the chip using a 0.18 μm CMOS process and developed a strategy to integrate the CMOS-MEA chip into the open microfluidic system within a larger overall effort to incorporate discrete CMOS sensors into microfluidic devices. Proof-of-concept experiments demonstrate the capability to perform electrophysiology and impedance spectroscopy of human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues, as well as electrochemical sensing of different analytes including hydrogen peroxide and epinephrine.

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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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