成纤维细胞图谱：组织中共享的和特定的细胞类型

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-04-02 DOI:10.1126/sciadv.ado0173

Kaidong Liu, Yanrui Cui, Huiming Han, Erliang Guo, Xingyang Shi, Kai Xiong, Nan Zhang, Songmei Zhai, Shaocong Sang, Mingyue Liu, Bo Chen, Yunyan Gu

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引用次数: 0

摘要

理解成纤维细胞的异质性取决于解码细胞亚型的复杂性、它们的起源、分布和与其他细胞的相互作用。在这里，我们整合了来自10个组织的73项研究的249,156个成纤维细胞，以呈现成纤维细胞的单细胞图谱。我们提供了18种成纤维细胞亚型的高分辨率分类。特别是，我们发现了一种以前未描述的细胞群，TSPAN8+染色质重塑成纤维细胞，其特征是具有组蛋白修饰和染色质重塑相关功能的基因的高表达。此外，在空间转录组数据和多重免疫荧光分析中可以检测到TSPAN8+染色质重塑成纤维细胞。与其他成纤维细胞亚型相比，TSPAN8+染色质重塑成纤维细胞在细胞分化和常驻成纤维细胞方面得分更高，主要通过配体VEGFA和受体F2R与内皮细胞和T细胞相互作用，其存在与预后不良相关。我们的分析全面定义了组织中成纤维细胞亚型的共同和特定特征，并提供了一个用户友好的数据门户——成纤维细胞图谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fibroblast atlas: Shared and specific cell types across tissues

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Fibroblast atlas: Shared and specific cell types across tissues

Understanding the heterogeneity of fibroblasts depends on decoding the complexity of cell subtypes, their origin, distribution, and interactions with other cells. Here, we integrated 249,156 fibroblasts from 73 studies across 10 tissues to present a single-cell atlas of fibroblasts. We provided a high-resolution classification of 18 fibroblast subtypes. In particular, we revealed a previously undescribed cell population, TSPAN8⁺ chromatin remodeling fibroblasts, characterized by high expression of genes with functions related to histone modification and chromatin remodeling. Moreover, TSPAN8⁺ chromatin remodeling fibroblasts were detectable in spatial transcriptome data and multiplexed immunofluorescence assays. Compared with other fibroblast subtypes, TSPAN8⁺ chromatin remodeling fibroblasts exhibited higher scores in cell differentiation and resident fibroblast, mainly interacting with endothelial cells and T cells through ligand VEGFA and receptor F2R, and their presence was associated with poor prognosis. Our analyses comprehensively defined the shared and specific characteristics of fibroblast subtypes across tissues and provided a user-friendly data portal, Fibroblast Atlas.

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.