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IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

International Journal of Clinical Practice Pub Date : 2025-04-03 DOI:10.1155/ijcp/5516061

Qutaiba Ababneh, Rahaf Alqazaq, Ziad Jaradat, Hala Khanjar

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引用次数: 0

摘要

许多微生物感染是由多个物种引起的。然而，人们对共存病原体之间的详细相互作用和竞争机制知之甚少。鲍曼不动杆菌是一种主要的院内病原体，经常参与混合感染。迄今为止，很少有研究关注鲍曼不动杆菌与其他常见共感染微生物之间的相互作用。在本研究中，我们旨在调查临床鲍曼不动杆菌分离株与肺炎克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌之间的种间竞争。2018 年至 2019 年期间，从约旦三家主要医院的 56 名患者中分离出了鲍曼尼甲菌。分离物来自各种临床标本，包括痰、伤口、血液和尿液。还使用了耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和肺炎双球菌的临床分离物。通过 168 项种间竞争实验研究了鲍曼不动杆菌分离株与其他每种细菌之间的相互作用，每项实验均重复进行。结果表明，鲍曼尼氏菌对肺炎双球菌的竞争优势明显，大大降低了肺炎双球菌的生长，降低的程度因鲍曼尼氏菌分离物的临床来源而异。此外，无论鲍曼不动杆菌的临床来源如何，金黄色葡萄球菌的生长都会下降，与肺炎克氏菌相比，金黄色葡萄球菌的生长下降幅度更大。另一方面，铜绿假单胞菌的存在导致鲍曼尼氏菌的生长显著下降，而铜绿假单胞菌与鲍曼尼氏菌共培养后的生长则显著增加。本研究提供的证据表明，与金黄色葡萄球菌和肺炎双球菌相比，鲍曼尼氏菌分离物具有竞争优势。相比之下，铜绿假单胞菌在鲍曼尼氏菌分离物中的竞争力更强。我们的研究结果为确定控制合并感染的潜在疗法提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Interspecies Competitive Interactions of Clinical Isolates of Acinetobacter baumannii With Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa

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Interspecies Competitive Interactions of Clinical Isolates of Acinetobacter baumannii With Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa

Many microbial infections are caused by multiple species. However, little is known about the detailed interactions and mechanisms underlying competition between coexisting pathogens. Acinetobacter baumannii is a major nosocomial pathogen that is often involved in mixed infections. To date, few studies have focused on the interactions between A. baumannii and other common coinfectious microbes. In this study, we aimed to investigate the interspecies competition among clinical A. baumannii isolates with Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. A. baumannii isolates were recovered from 56 patients in three major hospitals in Jordan between 2018 and 2019. The isolates were obtained from various clinical specimens, including sputum, wound, blood, and urine. Clinical isolates of methicillin-resistant S. aureus, P. aeruginosa, and K. pneumoniae were also used. Interactions between A. baumannii isolates and each of the other species were investigated through 168 interspecies competition assays, and each experiment was done in duplicate. The results revealed that A. baumannii outcompeted K. pneumoniae, significantly reducing its growth, with the degree of reduction varying depending on the clinical origin of the A. baumannii isolates. Moreover, the growth of S. aureus was decreased regardless of the A. baumannii clinical origin, with a higher reduction compared to K. pneumoniae. On the other hand, the presence of P. aeruginosa led to a significant decrease in the A. baumannii growth, while the growth of P. aeruginosa was significantly increased after coculture with A. baumannii. The present study provides evidence that the A. baumannii isolates have competitive advantages over both S. aureus and K. pneumoniae. In contrast, P. aeruginosa outcompetes A. baumannii isolates. Our findings provide new insights to identify potential therapeutics to control coinfections.

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来源期刊

International Journal of Clinical Practice 医学-医学：内科

CiteScore

5.30

自引率

0.00%

发文量

274

审稿时长

3-8 weeks

期刊介绍： IJCP is a general medical journal. IJCP gives special priority to work that has international appeal. IJCP publishes: Editorials. IJCP Editorials are commissioned. [Peer reviewed at the editor''s discretion] Perspectives. Most IJCP Perspectives are commissioned. Example. [Peer reviewed at the editor''s discretion] Study design and interpretation. Example. [Always peer reviewed] Original data from clinical investigations. In particular: Primary research papers from RCTs, observational studies, epidemiological studies; pre-specified sub-analyses; pooled analyses. [Always peer reviewed] Meta-analyses. [Always peer reviewed] Systematic reviews. From October 2009, special priority will be given to systematic reviews. [Always peer reviewed] Non-systematic/narrative reviews. From October 2009, reviews that are not systematic will be considered only if they include a discrete Methods section that must explicitly describe the authors'' approach. Special priority will, however, be given to systematic reviews. [Always peer reviewed] ''How to…'' papers. Example. [Always peer reviewed] Consensus statements. [Always peer reviewed] Short reports. [Always peer reviewed] Letters. [Peer reviewed at the editor''s discretion] International scope IJCP publishes work from investigators globally. Around 30% of IJCP articles list an author from the UK. Around 30% of IJCP articles list an author from the USA or Canada. Around 45% of IJCP articles list an author from a European country that is not the UK. Around 15% of articles published in IJCP list an author from a country in the Asia-Pacific region.