Qutaiba Ababneh, Rahaf Alqazaq, Ziad Jaradat, Hala Khanjar
{"title":"鲍曼不动杆菌与肺炎克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌临床分离株的种间竞争相互作用","authors":"Qutaiba Ababneh, Rahaf Alqazaq, Ziad Jaradat, Hala Khanjar","doi":"10.1155/ijcp/5516061","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Many microbial infections are caused by multiple species. However, little is known about the detailed interactions and mechanisms underlying competition between coexisting pathogens. <i>Acinetobacter baumannii</i> is a major nosocomial pathogen that is often involved in mixed infections. To date, few studies have focused on the interactions between <i>A</i>. <i>baumannii</i> and other common coinfectious microbes. In this study, we aimed to investigate the interspecies competition among clinical <i>A. baumannii</i> isolates with <i>Klebsiella pneumoniae</i>, <i>Staphylococcus aureus</i>, <i>and Pseudomonas aeruginosa</i>. <i>A. baumannii</i> isolates were recovered from 56 patients in three major hospitals in Jordan between 2018 and 2019. The isolates were obtained from various clinical specimens, including sputum, wound, blood, and urine. Clinical isolates of methicillin-resistant <i>S</i>. <i>aureus</i>, <i>P</i>. <i>aeruginosa</i>, <i>and K</i>. <i>pneumoniae</i> were also used. Interactions between <i>A</i>. <i>baumannii</i> isolates and each of the other species were investigated through 168 interspecies competition assays, and each experiment was done in duplicate. The results revealed that <i>A</i>. <i>baumannii</i> outcompeted <i>K</i>. <i>pneumoniae</i>, significantly reducing its growth, with the degree of reduction varying depending on the clinical origin of <i>the A</i>. <i>baumannii</i> isolates. Moreover, the growth of <i>S</i>. <i>aureus</i> was decreased regardless of the <i>A</i>. <i>baumannii</i> clinical origin, with a higher reduction compared to <i>K</i>. <i>pneumoniae</i>. On the other hand, the presence of <i>P</i>. <i>aeruginosa</i> led to a significant decrease in the <i>A</i>. <i>baumannii</i> growth, while the growth of <i>P</i>. <i>aeruginosa</i> was significantly increased after coculture with <i>A. baumannii</i>. The present study provides evidence that the <i>A</i>. <i>baumannii</i> isolates have competitive advantages over both <i>S</i>. <i>aureus</i> and <i>K</i>. <i>pneumoniae</i>. In contrast, <i>P</i>. <i>aeruginosa</i> outcompetes <i>A</i>. <i>baumannii</i> isolates. Our findings provide new insights to identify potential therapeutics to control coinfections.</p>\n </div>","PeriodicalId":13782,"journal":{"name":"International Journal of Clinical Practice","volume":"2025 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/ijcp/5516061","citationCount":"0","resultStr":"{\"title\":\"Interspecies Competitive Interactions of Clinical Isolates of Acinetobacter baumannii With Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa\",\"authors\":\"Qutaiba Ababneh, Rahaf Alqazaq, Ziad Jaradat, Hala Khanjar\",\"doi\":\"10.1155/ijcp/5516061\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>Many microbial infections are caused by multiple species. However, little is known about the detailed interactions and mechanisms underlying competition between coexisting pathogens. <i>Acinetobacter baumannii</i> is a major nosocomial pathogen that is often involved in mixed infections. To date, few studies have focused on the interactions between <i>A</i>. <i>baumannii</i> and other common coinfectious microbes. In this study, we aimed to investigate the interspecies competition among clinical <i>A. baumannii</i> isolates with <i>Klebsiella pneumoniae</i>, <i>Staphylococcus aureus</i>, <i>and Pseudomonas aeruginosa</i>. <i>A. baumannii</i> isolates were recovered from 56 patients in three major hospitals in Jordan between 2018 and 2019. The isolates were obtained from various clinical specimens, including sputum, wound, blood, and urine. Clinical isolates of methicillin-resistant <i>S</i>. <i>aureus</i>, <i>P</i>. <i>aeruginosa</i>, <i>and K</i>. <i>pneumoniae</i> were also used. Interactions between <i>A</i>. <i>baumannii</i> isolates and each of the other species were investigated through 168 interspecies competition assays, and each experiment was done in duplicate. The results revealed that <i>A</i>. <i>baumannii</i> outcompeted <i>K</i>. <i>pneumoniae</i>, significantly reducing its growth, with the degree of reduction varying depending on the clinical origin of <i>the A</i>. <i>baumannii</i> isolates. Moreover, the growth of <i>S</i>. <i>aureus</i> was decreased regardless of the <i>A</i>. <i>baumannii</i> clinical origin, with a higher reduction compared to <i>K</i>. <i>pneumoniae</i>. On the other hand, the presence of <i>P</i>. <i>aeruginosa</i> led to a significant decrease in the <i>A</i>. <i>baumannii</i> growth, while the growth of <i>P</i>. <i>aeruginosa</i> was significantly increased after coculture with <i>A. baumannii</i>. The present study provides evidence that the <i>A</i>. <i>baumannii</i> isolates have competitive advantages over both <i>S</i>. <i>aureus</i> and <i>K</i>. <i>pneumoniae</i>. In contrast, <i>P</i>. <i>aeruginosa</i> outcompetes <i>A</i>. <i>baumannii</i> isolates. 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Interspecies Competitive Interactions of Clinical Isolates of Acinetobacter baumannii With Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa
Many microbial infections are caused by multiple species. However, little is known about the detailed interactions and mechanisms underlying competition between coexisting pathogens. Acinetobacter baumannii is a major nosocomial pathogen that is often involved in mixed infections. To date, few studies have focused on the interactions between A. baumannii and other common coinfectious microbes. In this study, we aimed to investigate the interspecies competition among clinical A. baumannii isolates with Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. A. baumannii isolates were recovered from 56 patients in three major hospitals in Jordan between 2018 and 2019. The isolates were obtained from various clinical specimens, including sputum, wound, blood, and urine. Clinical isolates of methicillin-resistant S. aureus, P. aeruginosa, and K. pneumoniae were also used. Interactions between A. baumannii isolates and each of the other species were investigated through 168 interspecies competition assays, and each experiment was done in duplicate. The results revealed that A. baumannii outcompeted K. pneumoniae, significantly reducing its growth, with the degree of reduction varying depending on the clinical origin of the A. baumannii isolates. Moreover, the growth of S. aureus was decreased regardless of the A. baumannii clinical origin, with a higher reduction compared to K. pneumoniae. On the other hand, the presence of P. aeruginosa led to a significant decrease in the A. baumannii growth, while the growth of P. aeruginosa was significantly increased after coculture with A. baumannii. The present study provides evidence that the A. baumannii isolates have competitive advantages over both S. aureus and K. pneumoniae. In contrast, P. aeruginosa outcompetes A. baumannii isolates. Our findings provide new insights to identify potential therapeutics to control coinfections.
期刊介绍：
IJCP is a general medical journal. IJCP gives special priority to work that has international appeal.
IJCP publishes:
Editorials. IJCP Editorials are commissioned. [Peer reviewed at the editor''s discretion]
Perspectives. Most IJCP Perspectives are commissioned. Example. [Peer reviewed at the editor''s discretion]
Study design and interpretation. Example. [Always peer reviewed]
Original data from clinical investigations. In particular: Primary research papers from RCTs, observational studies, epidemiological studies; pre-specified sub-analyses; pooled analyses. [Always peer reviewed]
Meta-analyses. [Always peer reviewed]
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''How to…'' papers. Example. [Always peer reviewed]
Consensus statements. [Always peer reviewed] Short reports. [Always peer reviewed]
Letters. [Peer reviewed at the editor''s discretion]
International scope
IJCP publishes work from investigators globally. Around 30% of IJCP articles list an author from the UK. Around 30% of IJCP articles list an author from the USA or Canada. Around 45% of IJCP articles list an author from a European country that is not the UK. Around 15% of articles published in IJCP list an author from a country in the Asia-Pacific region.
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