a型肉毒毒素对大鼠偏头痛模型三叉神经节核苷酸结合寡聚化结构域样受体3炎性体的影响

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2025-04-02 DOI:10.1016/j.cyto.2025.156934

Jun Shen , Xiaofeng Zhu , Lei Xia , Jin Shang , Ming Wei , Qiu Han

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引用次数: 0

摘要

A型肉毒毒素（BTX-A）已被用于预防和治疗慢性偏头痛，但其具体机制尚不完全清楚。目的探讨BTX-A对偏头痛模型大鼠三叉神经节核苷结合寡聚化结构域样受体蛋白3 （NLRP3）炎性体和白细胞介素-1β (IL-1β)的影响。方法健康成年雄性SD大鼠随机分为各组。采用Von Frey纤维检测大鼠眶周疼痛区。采用免疫印迹法和免疫荧光法检测大鼠三叉神经节中NLRP3炎性体和IL-1β的表达。结果偏头痛模型组大鼠眶周疼痛面积明显低于Sham组，差异有统计学意义（p <； 0.05）。与Sham组比较，偏头痛模型组NLRP3、pro-caspase-1、caspase-1、成熟IL-1β表达均显著升高，差异有统计学意义（p <； 0.05）。与IA对照组相比，5 U/kg BTX组和10 U/kg BTX- a组NLRP3、pro-caspase-1、caspase-1和成熟IL-1β的表达量显著降低（p <； 0.05）。结论btx - a抑制大鼠偏头痛模型三叉神经节NLRP3炎性体和成熟IL-1β的合成。其对三叉神经初级伤害神经元炎症的抑制作用可能是其预防偏头痛的重要机制之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effects of botulinum toxin type a on nucleotide binding oligomerization domain-like receptor 3 inflammasome in trigeminal ganglion of a rat migraine model

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Effects of botulinum toxin type a on nucleotide binding oligomerization domain-like receptor 3 inflammasome in trigeminal ganglion of a rat migraine model

Background

Botulinum toxin type A (BTX-A) has been used in the prevention and treatment of chronic migraine, but the detailed mechanism was not clear completely.

Objective

The effects of BTX-A on nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome and interleukin-1 beta (IL-1β) were explored in the trigeminal ganglion of migraine model rats.

Methods

Healthy adult male Sprague-Dawley (SD) rats were randomly divided into groups. Von Frey fiber filaments were used to detect the periorbital pain area of rats. The immunoblotting and Immunofluorescence were used to detect the expression of NLRP3 inflammasome and IL-1β in the trigeminal ganglia of rats.

Results

The periorbital pain area of rats in the migraine model group was significantly lower than that of the Sham group, and the difference was statistically significant (p < 0.05). Compared with the Sham group, the expressions of NLRP3, pro-caspase-1, caspase-1 and mature IL-1β in the migraine model group were significantly increased, and the difference was statistically significant (p < 0.05). Compared with the IA control group, the expressions of NLRP3, pro-caspase-1, caspase-1 and mature IL-1β in 5 U/kg BTX and 10 U/kg BTX-A group were significantly reduced (p < 0.05).

Conclusion

BTX-A inhibits the synthesis of NLRP3 inflammasome and mature IL-1β in the trigeminal ganglion from rat migraine models. Its inhibitory effect on the inflammation of the primary nociceptive neurons of the trigeminal nerve may be one of its important mechanisms for the prevention of migraine.

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.