肺内转移的新原因和评估

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-04-03 DOI:10.1016/j.bbrep.2025.102004

Ming Cheng, Shujun Shao, Wei Xu, Dazhi Liu

{"title":"肺内转移的新原因和评估","authors":"Ming Cheng, Shujun Shao, Wei Xu, Dazhi Liu","doi":"10.1016/j.bbrep.2025.102004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>This study utilized next-generation sequencing (NGS) to analyze genetic information and gene mutation-related loci in ground glass nodules (GGN) with multiple (≥2) lesions. Pathological findings were then correlated to distinguish between multiple primary lung cancers (MPLC) and pulmonary metastasis (PM).</div></div><div><h3>Methods</h3><div>A cohort of 20 individuals who underwent surgical resection for ground glass nodules (GGN) was included. Final diagnosis and restaging were determined based on the analysis of clinical characteristics and NGS single-target genetic detection.</div></div><div><h3>Results</h3><div>Histopathological, immunohistochemical staining, and NGS analyses identified 48 tissue samples from 20 cases of multiple malignant nodules. A total of 66 gene mutations were identified, with four cases classified as PM. Notably, four patients with intrapulmonary metastases exhibited concurrent mutations in the epidermal growth factor receptor (EGFR) (50 %) and Kirsten ratsarcoma viral oncogene homolog (KRAS). Comparatively, the prevalence of EGFR mutations in PM patients was significantly higher than that in primary lesions.</div></div><div><h3>Conclusion</h3><div>Genomic analysis of multiple lung adenocarcinomas enables the determination of the clonal status of tumor cells across various lesions. When gene mutation sites in multiple lesions are identical and mutation abundance is significantly elevated, early intrapulmonary metastasis may be diagnosed.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"42 ","pages":"Article 102004"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Novel causes and assessments of intrapulmonary metastasis\",\"authors\":\"Ming Cheng, Shujun Shao, Wei Xu, Dazhi Liu\",\"doi\":\"10.1016/j.bbrep.2025.102004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>This study utilized next-generation sequencing (NGS) to analyze genetic information and gene mutation-related loci in ground glass nodules (GGN) with multiple (≥2) lesions. Pathological findings were then correlated to distinguish between multiple primary lung cancers (MPLC) and pulmonary metastasis (PM).</div></div><div><h3>Methods</h3><div>A cohort of 20 individuals who underwent surgical resection for ground glass nodules (GGN) was included. Final diagnosis and restaging were determined based on the analysis of clinical characteristics and NGS single-target genetic detection.</div></div><div><h3>Results</h3><div>Histopathological, immunohistochemical staining, and NGS analyses identified 48 tissue samples from 20 cases of multiple malignant nodules. A total of 66 gene mutations were identified, with four cases classified as PM. Notably, four patients with intrapulmonary metastases exhibited concurrent mutations in the epidermal growth factor receptor (EGFR) (50 %) and Kirsten ratsarcoma viral oncogene homolog (KRAS). Comparatively, the prevalence of EGFR mutations in PM patients was significantly higher than that in primary lesions.</div></div><div><h3>Conclusion</h3><div>Genomic analysis of multiple lung adenocarcinomas enables the determination of the clonal status of tumor cells across various lesions. When gene mutation sites in multiple lesions are identical and mutation abundance is significantly elevated, early intrapulmonary metastasis may be diagnosed.</div></div>\",\"PeriodicalId\":8771,\"journal\":{\"name\":\"Biochemistry and Biophysics Reports\",\"volume\":\"42 \",\"pages\":\"Article 102004\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry and Biophysics Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405580825000913\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580825000913","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

本研究利用新一代测序技术（NGS）分析了伴有多个（≥2个）病变的磨玻璃结节（GGN）的遗传信息和基因突变相关位点。然后将病理结果与多原发肺癌（MPLC）和肺转移（PM）区分开来。方法对20例手术切除磨砂玻璃结节（GGN）患者进行回顾性分析。根据临床特征分析和NGS单靶点基因检测确定最终诊断和再手术。结果20例多发性恶性结节48例组织病理、免疫组化染色及NGS鉴定。共鉴定出66例基因突变，其中4例为PM。值得注意的是，4例肺内转移患者在表皮生长因子受体（EGFR）（50%）和克尔斯滕肉瘤病毒癌基因同源物（KRAS）中同时发生突变。相比之下，PM患者中EGFR突变的发生率明显高于原发病变。结论通过对多种肺腺癌的基因组分析，可以确定不同病变中肿瘤细胞的克隆状态。当多个病变的基因突变位点相同且突变丰度显著升高时，可早期诊断肺内转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Novel causes and assessments of intrapulmonary metastasis

Background

This study utilized next-generation sequencing (NGS) to analyze genetic information and gene mutation-related loci in ground glass nodules (GGN) with multiple (≥2) lesions. Pathological findings were then correlated to distinguish between multiple primary lung cancers (MPLC) and pulmonary metastasis (PM).

Methods

A cohort of 20 individuals who underwent surgical resection for ground glass nodules (GGN) was included. Final diagnosis and restaging were determined based on the analysis of clinical characteristics and NGS single-target genetic detection.

Results

Histopathological, immunohistochemical staining, and NGS analyses identified 48 tissue samples from 20 cases of multiple malignant nodules. A total of 66 gene mutations were identified, with four cases classified as PM. Notably, four patients with intrapulmonary metastases exhibited concurrent mutations in the epidermal growth factor receptor (EGFR) (50 %) and Kirsten ratsarcoma viral oncogene homolog (KRAS). Comparatively, the prevalence of EGFR mutations in PM patients was significantly higher than that in primary lesions.

Conclusion

Genomic analysis of multiple lung adenocarcinomas enables the determination of the clonal status of tumor cells across various lesions. When gene mutation sites in multiple lesions are identical and mutation abundance is significantly elevated, early intrapulmonary metastasis may be diagnosed.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.