己糖激酶2和单细胞测序在胃癌诊断中的应用。

IF 2.7 4区 医学 Q2 HEALTH CARE SCIENCES & SERVICES
Risk Management and Healthcare Policy Pub Date : 2025-03-25 eCollection Date: 2025-01-01 DOI:10.2147/RMHP.S510123
Peiyu Qian, Jie Sun, Zhenya Zhao, Peihua Lu
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引用次数: 0

摘要

目的:由于胃癌的高患病率和死亡率,胃癌是一个重大的全球健康挑战,这主要归因于当前筛查方法的局限性,如内窥镜检查,阻碍了早期诊断。本研究提出了一种通过识别洗胃液中脱落的肿瘤细胞进行早期检测的创新方法,旨在克服与患者依从性和内窥镜医师专业知识差异相关的挑战。方法:利用代谢标志物己糖激酶2 (HK2)检测胃灌洗液中糖酵解活性增高的脱落肿瘤细胞。利用单细胞测序(SCS),特别是通过全基因组拷贝数变异分析,进一步验证了这些hk2阳性、高糖酵解肿瘤细胞的恶性性。结果:共评估了60例个体,包括10例胃癌患者(IA期9例,IIA期1例),26例癌前病变患者,15例胃良性疾病患者,9例健康对照。HK2检测显示对胃癌IA期和IIA期的诊断敏感性为80%,在区分良性状况和健康对照方面的诊断特异性为96%。重要的是,该检测对严重不典型增生病例的敏感性为57%,强调了其早期胃癌检测和预防性诊断的潜力。结论:本研究强调了一种新的基于洗胃的HK2检测方法的可行性,并辅以SCS进行恶性肿瘤确认,作为一种高度准确的胃癌早期检测方法。这种方法为传统胃镜检查提供了一种有希望的替代方法,特别是对于早期疾病,有可能提高检出率并改善患者的预后。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Early Detection of Malignant Cells in Gastric Lavage via Hexokinase 2 and Single-Cell Sequencing for Gastric Cancer Diagnosis.

Objective: Gastric cancer represents a significant global health challenge due to its high prevalence and mortality rates, largely attributed to the limitations of current screening methods, such as endoscopy, which impede early diagnosis. This study presents an innovative method for early detection by identifying exfoliated tumor cells in gastric lavage, aiming to overcome challenges related to patient compliance and the variability in endoscopist expertise.

Methods: Hexokinase 2 (HK2), a metabolic marker, was utilized to identify exfoliated tumor cells with heightened glycolytic activity in gastric lavage fluid. The malignancy of these HK2-positive, high-glycolytic tumor cells was further validated using single-cell sequencing (SCS), specifically through genome-wide copy number variation analysis.

Results: A total of 60 individuals were assessed, including 10 patients with gastric cancer (9 at stage IA and 1 at stage IIA), 26 patients with precancerous lesions, 15 patients with benign gastric conditions, and 9 healthy controls. The HK2 assay demonstrated an 80% diagnostic sensitivity for stages IA and IIA of gastric cancer and a 96% diagnostic specificity in distinguishing benign conditions from healthy controls. Importantly, the assay exhibited 57% sensitivity for cases of severe dysplasia, underscoring its potential for early gastric cancer detection and preventive diagnostics.

Conclusion: The study highlights the feasibility of a novel gastric lavage-based HK2 assay, complemented by SCS for malignancy confirmation, as a highly accurate method for the early detection of gastric cancer. This approach offers a promising alternative to traditional gastroscopy, particularly for early-stage disease, potentially enhancing detection rates and improving patient outcomes.

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来源期刊
Risk Management and Healthcare Policy
Risk Management and Healthcare Policy Medicine-Public Health, Environmental and Occupational Health
CiteScore
6.20
自引率
2.90%
发文量
242
审稿时长
16 weeks
期刊介绍: Risk Management and Healthcare Policy is an international, peer-reviewed, open access journal focusing on all aspects of public health, policy and preventative measures to promote good health and improve morbidity and mortality in the population. Specific topics covered in the journal include: Public and community health Policy and law Preventative and predictive healthcare Risk and hazard management Epidemiology, detection and screening Lifestyle and diet modification Vaccination and disease transmission/modification programs Health and safety and occupational health Healthcare services provision Health literacy and education Advertising and promotion of health issues Health economic evaluations and resource management Risk Management and Healthcare Policy focuses on human interventional and observational research. The journal welcomes submitted papers covering original research, clinical and epidemiological studies, reviews and evaluations, guidelines, expert opinion and commentary, and extended reports. Case reports will only be considered if they make a valuable and original contribution to the literature. The journal does not accept study protocols, animal-based or cell line-based studies.
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