积雪草提取物-3增强HEK293肾细胞的抗氧化能力和凋亡细胞死亡。

IF 1.7 3区 农林科学 Q4 CHEMISTRY, MEDICINAL
Dhaneshree B Naidoo, Alisa Phulukdaree, Anil Armichund Chuturgoon, Vikash Sewram
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引用次数: 0

摘要

传统药用植物积雪草(Centella asiatica)由于其广泛的治疗特性,通常用于中国和阿育吠陀医学。在此之前,我们用硅柱对乙醇化的积雪草叶提取物进行分离,得到C3部分。我们研究了C3对人胚胎肾(HEK293)细胞的抗氧化和抗增殖作用。在HEK293细胞中,评估C3细胞毒性(活力测定;24小时;[0.2 ~ 3 mg/mL]),测定一半最大抑制浓度(IC50)。评估丙二醛(MDA)、乳酸脱氢酶(LDH)(分光光度法)、线粒体去极化(Δψm)、细胞内活性氧(流式细胞术)、谷胱甘肽(GSH)、氧化谷胱甘肽(GSSG)浓度、半胱甘肽酶活性、ATP水平(光度法)和DNA片段化(SCGE法)。western blotting检测蛋白表达。采用qPCR法测定基因表达。C3使HEK293细胞活力呈剂量依赖性降低。C3显著提高了MDA、Δψm、LDH、caspase活性和DNA断裂度(P < .0004)。C3显著降低抗氧化(Nrf-2、GPx、SOD、CAT)基因表达(P < 0.001),显著提高了GSH、GSSG浓度和Nrf-2蛋白表达(P < 0.001)。C3降低Bax和Bcl-2蛋白表达(P < 0.05)。C3组c-myc基因表达量显著升高,OGG-1基因表达量显著降低(P < 0.05)。在HEK293细胞中,C3降低抗氧化基因表达,增加抗氧化水平,增强抗增殖作用,提示高浓度C3对肾细胞具有潜在毒性,因此引起对其人类使用的关注。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Centella Asiatica Fraction-3 Enhances Antioxidant Capacity and Apoptotic Cell Death in HEK293 Kidney Cells.

The traditional medicinal plant Centella asiatica is commonly used in Chinese and Ayurvedic medicine due to its vast range of therapeutic properties. Previously, the ethanolic C. asiatica leaf extract was subjected to silica column fractionation, and the C3 fraction was obtained. We investigated the antioxidant and anti-proliferative effects of C3 in human embryonic kidney (HEK293) cells. In HEK293 cells, C3 cytotoxicity was assessed (viability assay; 24 h; [0.2-3 mg/mL]), and a half maximal inhibitory concentration (IC50) was determined. Malondialdehyde (MDA), lactate dehydrogenase (LDH) (spectrophotometry), mitochondrial depolarization (Δψm), intracellular reactive oxygen species (flow cytometry), glutathione (GSH), oxidized glutathione (GSSG) concentrations, caspase activities, ATP levels (luminometry), and fragmentation of DNA (SCGE assay) were evaluated. Protein expressions were assessed by western blotting. Gene expressions were quantified by qPCR. Cell viability in HEK293 cells was decreased in a dose-dependent manner by C3. MDA, Δψm, LDH, caspase activities, and DNA fragmentation (P < .0004) were significantly increased by C3. Nuclear factor erythroid 2-related factor 2 (Nrf-2) protein expression, GSH, and GSSG concentrations were increased, whereas antioxidant (Nrf-2, GPx, SOD, and CAT) gene expression was significantly decreased by C3 (P < .001). C3 decreased both Bax and Bcl-2 protein expression (P < .03). Gene expression of c-myc was significantly increased, whereas OGG-1 was significantly reduced by C3 (P < .05). C3 reduced antioxidant gene expression, increased antioxidant levels, and elevated anti-proliferative effects in HEK293 cells, suggesting that high concentrations of C3 are potentially toxic to kidney cells, thus rendering cause for concern with its human use.

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来源期刊
Journal of medicinal food
Journal of medicinal food 医学-食品科技
CiteScore
4.50
自引率
0.00%
发文量
154
审稿时长
4.5 months
期刊介绍: Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.
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