{"title":"Isatis glauca Aucher Ex Boiss。无性系种群。靛蓝提取物作为酶抑制剂的生物化学研究","authors":"Beyza Sevdenur Nezir, Edanur Dasdemir, Tuba Aydın, Ruya Saglamtas, Elife Kaya, Fevzi Ozgokce","doi":"10.1002/slct.202405991","DOIUrl":null,"url":null,"abstract":"<p><i>Isatis glauca</i> Aucher ex Boiss. subsp. <i>glauca</i> is a species of indigo that grows naturally in Türkiye. In this study, the in vitro biological activities of <i>I. glauca</i> were evaluated for the first time. The inhibitory effects of <i>I. glauca</i>’s methanol, ethanol, dichloromethane/methanol (50:50), acetone, ethyl acetate, dichloromethane, and <i>n</i>-hexane extracts were investigated on the enzymes human carbonic anhydrase I (hCAI), human carbonic anhydrase II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase. IC<sub>50</sub> values were calculated to determine the inhibitory effect of the extracts. Among the extracts, the ones with the strongest inhibitory activity against hCAI, hCAII, AChE, BChE, and α-glucosidase were found to be ethyl acetate extract (IC<sub>50</sub>: 75.77 mg/mL), methanol extract (IC<sub>50</sub>: 102.89 mg/mL), <i>n</i>-hexane extract (IC<sub>50</sub>: 61.11 mg/mL), ethyl acetate extract (IC<sub>50</sub>: 22.79 mg/mL), and ethyl acetate extract (IC<sub>50</sub>: 77.12 mg/mL), respectively. The study findings suggest that all extracts of <i>I. glauca</i> may have promising therapeutic properties. In particular, it can be suggested that these extracts have the potential to be used in the treatment of diseases or the alleviation of symptoms. However, additional research and clinical trials are required to determine its efficacy. The isolation of <i>I. glauca</i> phytochemicals should be addressed in future studies.</p>","PeriodicalId":146,"journal":{"name":"ChemistrySelect","volume":"10 13","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isatis glauca Aucher Ex Boiss. Subsp. Glauca (Indigo) Extracts as Enzyme Inhibitors: A Biochemical Study\",\"authors\":\"Beyza Sevdenur Nezir, Edanur Dasdemir, Tuba Aydın, Ruya Saglamtas, Elife Kaya, Fevzi Ozgokce\",\"doi\":\"10.1002/slct.202405991\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Isatis glauca</i> Aucher ex Boiss. subsp. <i>glauca</i> is a species of indigo that grows naturally in Türkiye. In this study, the in vitro biological activities of <i>I. glauca</i> were evaluated for the first time. The inhibitory effects of <i>I. glauca</i>’s methanol, ethanol, dichloromethane/methanol (50:50), acetone, ethyl acetate, dichloromethane, and <i>n</i>-hexane extracts were investigated on the enzymes human carbonic anhydrase I (hCAI), human carbonic anhydrase II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase. IC<sub>50</sub> values were calculated to determine the inhibitory effect of the extracts. Among the extracts, the ones with the strongest inhibitory activity against hCAI, hCAII, AChE, BChE, and α-glucosidase were found to be ethyl acetate extract (IC<sub>50</sub>: 75.77 mg/mL), methanol extract (IC<sub>50</sub>: 102.89 mg/mL), <i>n</i>-hexane extract (IC<sub>50</sub>: 61.11 mg/mL), ethyl acetate extract (IC<sub>50</sub>: 22.79 mg/mL), and ethyl acetate extract (IC<sub>50</sub>: 77.12 mg/mL), respectively. The study findings suggest that all extracts of <i>I. glauca</i> may have promising therapeutic properties. In particular, it can be suggested that these extracts have the potential to be used in the treatment of diseases or the alleviation of symptoms. However, additional research and clinical trials are required to determine its efficacy. The isolation of <i>I. glauca</i> phytochemicals should be addressed in future studies.</p>\",\"PeriodicalId\":146,\"journal\":{\"name\":\"ChemistrySelect\",\"volume\":\"10 13\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemistrySelect\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/slct.202405991\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemistrySelect","FirstCategoryId":"92","ListUrlMain":"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/slct.202405991","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Isatis glauca Aucher Ex Boiss. Subsp. Glauca (Indigo) Extracts as Enzyme Inhibitors: A Biochemical Study
Isatis glauca Aucher ex Boiss. subsp. glauca is a species of indigo that grows naturally in Türkiye. In this study, the in vitro biological activities of I. glauca were evaluated for the first time. The inhibitory effects of I. glauca’s methanol, ethanol, dichloromethane/methanol (50:50), acetone, ethyl acetate, dichloromethane, and n-hexane extracts were investigated on the enzymes human carbonic anhydrase I (hCAI), human carbonic anhydrase II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase. IC50 values were calculated to determine the inhibitory effect of the extracts. Among the extracts, the ones with the strongest inhibitory activity against hCAI, hCAII, AChE, BChE, and α-glucosidase were found to be ethyl acetate extract (IC50: 75.77 mg/mL), methanol extract (IC50: 102.89 mg/mL), n-hexane extract (IC50: 61.11 mg/mL), ethyl acetate extract (IC50: 22.79 mg/mL), and ethyl acetate extract (IC50: 77.12 mg/mL), respectively. The study findings suggest that all extracts of I. glauca may have promising therapeutic properties. In particular, it can be suggested that these extracts have the potential to be used in the treatment of diseases or the alleviation of symptoms. However, additional research and clinical trials are required to determine its efficacy. The isolation of I. glauca phytochemicals should be addressed in future studies.
期刊介绍:
ChemistrySelect is the latest journal from ChemPubSoc Europe and Wiley-VCH. It offers researchers a quality society-owned journal in which to publish their work in all areas of chemistry. Manuscripts are evaluated by active researchers to ensure they add meaningfully to the scientific literature, and those accepted are processed quickly to ensure rapid online publication.