{"title":"Real-Time PCR检测口腔鳞状细胞癌和口腔扁平苔藓中核（∆EX3）和细胞质（2B） survivin表达的比较研究","authors":"Maryam Amirchaghmaghi, Atessa Pakfetrat, Nooshin Mohtasham, Farnaz Mohajertehran, Mohammad Taghi shakeri, Elahe Vazavandi","doi":"10.1002/cre2.70123","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>This cross-sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real-time polymerase chain reaction. The data were analyzed using SPSS 20.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The expression of survivin-∆EX3 and survivin-2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (<i>p</i> < 0.05). The mean expression of survivin-∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, <i>p</i> < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (<i>p</i> < 0.05). There was also a significant correlation between gene expressions (<i>p</i> < 0.001).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The increased expression of survivin-∆EX3 and survivin-2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70123","citationCount":"0","resultStr":"{\"title\":\"Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real-Time PCR\",\"authors\":\"Maryam Amirchaghmaghi, Atessa Pakfetrat, Nooshin Mohtasham, Farnaz Mohajertehran, Mohammad Taghi shakeri, Elahe Vazavandi\",\"doi\":\"10.1002/cre2.70123\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>This cross-sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real-time polymerase chain reaction. The data were analyzed using SPSS 20.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The expression of survivin-∆EX3 and survivin-2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (<i>p</i> < 0.05). The mean expression of survivin-∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, <i>p</i> < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (<i>p</i> < 0.05). There was also a significant correlation between gene expressions (<i>p</i> < 0.001).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The increased expression of survivin-∆EX3 and survivin-2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.</p>\\n </section>\\n </div>\",\"PeriodicalId\":10203,\"journal\":{\"name\":\"Clinical and Experimental Dental Research\",\"volume\":\"11 1\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70123\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Dental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70123\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70123","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real-Time PCR
Objective
Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.
Materials and Methods
This cross-sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real-time polymerase chain reaction. The data were analyzed using SPSS 20.
Results
The expression of survivin-∆EX3 and survivin-2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (p < 0.05). The mean expression of survivin-∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, p < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (p < 0.05). There was also a significant correlation between gene expressions (p < 0.001).
Conclusion
The increased expression of survivin-∆EX3 and survivin-2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.
期刊介绍：
Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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