具有药物缓释和骨免疫调节作用的易溶纳米复合水凝胶支架促进骨再生

IF 8.2 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Fang Zhao, Yuxuan Su, Hong Liu, Yong Zhao, Liao Zhang, Nanshan Zhuge, Peng Zhao, Zhaoliang Ning, Qi Kang and Dongxu Liu*, 
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引用次数: 0

摘要

无外源性细胞的高质量骨缺损修复仍然是世界范围内的主要临床挑战。在此,我们将阿司匹林(ASA)负载的介孔二氧化硅纳米颗粒(MSNs)加入到genipin交联的盐酸壳聚糖(CSH)中,制备了纳米复合水凝胶支架(ASA/MSNs/CSH)。所得到的支架被设计用于在骨再生过程中提供免疫调节支持。将负载asa的msn包封在CSH中,形成一种复合水凝胶,能够持续释放药物超过35天。该复合水凝胶能够满足物理化学性质、机械强度、生物相容性和细胞亲和力的关键标准。研究表明,该支架在体外可通过减少炎症和诱导巨噬细胞向m2极化表型转变来创造有益的免疫微环境。支架还能促进骨髓间充质间质细胞的成骨,如增强碱性磷酸酶活性和钙结节的形成。同时,通过Western blot分析发现TGF-β/Smad通路是一个重要的调控机制。此外,在大鼠颅骨中建立了临界尺寸缺陷模型,结果表明ASA/MSNs/CSH纳米复合支架具有良好的生物相容性、良好的抗炎作用和良好的骨再生能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Facile Nanocomposite Hydrogel Scaffold with Sustained Drug Release and Osteo-Immunomodulatory Effects to Enhance Bone Regeneration

Facile Nanocomposite Hydrogel Scaffold with Sustained Drug Release and Osteo-Immunomodulatory Effects to Enhance Bone Regeneration

High-quality repair of critical bone defects without exogenous cells remains a major clinical challenge worldwide. Herein, we fabricated a nanocomposite hydrogel scaffold (ASA/MSNs/CSH) by incorporating aspirin (ASA)-loaded mesoporous silica nanoparticles (MSNs) into genipin-cross-linked chitosan hydrochloride (CSH). The resulting scaffold was designed to provide immunomodulatory support during the process of bone regeneration. ASA-loaded MSNs were encapsulated in CSH, forming a composite hydrogel capable of sustained drug release for over 35 days. This composite hydrogel was able to meet key criteria for physicochemical properties, mechanical strength, biocompatibility, and cell affinity. The study showed that the scaffolds could create a beneficial immune microenvironment through reducing inflammation and inducing macrophages toward M2-polarized phenotype in vitro. The scaffold also enhanced the osteogenesis of bone marrow mesenchymal stromal cells, as demonstrated by enhancing the alkaline phosphatase activity and the formation of calcium nodules. Meanwhile, the TGF-β/Smad pathway was identified as an important regulatory mechanism via Western blot analysis. Moreover, the critical size defect models were established in rat skulls, and the results demonstrated that the ASA/MSNs/CSH nanocomposite scaffolds exhibited adequate biocompatibility, superior anti-inflammatory effect, and an admirable capacity for bone regeneration in vivo.

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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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