揭示链霉菌对番石榴根结线虫的毒作用

IF 4.1 1区 农林科学 Q1 ENTOMOLOGY
Janani Mani, Jayakanthan Mannu, Prabhu Somasundaram, Devrajan Kandasamy, Thamizh Vendan Ragupathy, Harish Sankarasubramanian, Seenivasan Nagachandrabose
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引用次数: 0

摘要

由于肠根结线虫的入侵,印度番石榴生产面临着重大挑战。利用产生代谢物的链霉菌(Streptomyces sp .)进行生物防治,是控制土壤和根系中线虫种群的较好选择。从抑制线虫的番石榴根际分离的原生罗氏链霉菌与其他原生种相比,对肠芽胞杆菌的卵孵化率(100%)和幼虫死亡率(100%)有完全的抑制作用。此外,利用气相色谱/质谱(GC/MS)分析了罗氏菌产生的次生代谢产物,并与大肠杆菌的关键蛋白Me col1(胶原基因)进行了分子对接实验。其中,氧化苦参碱、麻子糖、2(3H)-呋喃酮、5-己基二氢、2-壬烷酮和环己烷等生物活性物质均具有杀线虫活性。其中,氧化苦参碱(−6.7 kcal/mol)、紫糖糖(−6.5 kcal/mol)和2(3H)-呋喃酮(−4.4 kcal/mol)对Me col1的结合亲和力最高。通过温室试验,研究了罗氏葡萄球菌(S. rochei GA)、链霉菌(Streptomyces spp. GHS-3)和GHRS-5无细胞培养滤液提取物对接种肠芽孢杆菌的番石榴幼苗的线虫抑制作用。经rochei GA处理的番石榴植株,寄生虫卵数减少79.1%,植株生长促进率提高75%。本研究强调了s.rochei GA生产的生物分子的线虫毒性潜力,作为一种有希望的替代人工合成的杀线虫剂来管理enterolobii。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling the nematotoxic effect of Streptomyces spp. against the root-knot nematode, Meloidogyne enterolobii in guava

Guava production in India faces significant challenges due to the invasion of the root-knot nematode Meloidogyne enterolobii. Biological control using metabolite-producing Streptomyces spp. offers a better alternative to synthetic nematicides for managing nematode populations in soil and roots. The native Streptomyces rochei isolated from nematode suppressive guava rhizosphere demonstrated complete inhibition of M. enterolobii egg hatching (100%) and juvenile mortality (100%) compared to other native species. Further, secondary metabolites produced by S. rochei were profiled using gas chromatography/mass spectrometry (GC/MS) analysis and molecular docking experiments were carried out with the key protein Me col-1 (collagen gene) of M. enterolobii. Notably, bioactive compounds of S. rochei such as oxymatrine, melezitose, 2(3H)-furanone, 5-hexyldihydro, 2-nonadecanone 2, and cyclohexane exhibited nematicidal activities. Among them, oxymatrine (− 6.7 kcal/mol), melezitose (− 6.5 kcal/mol), and 2(3H)-furanone (−4.4 kcal/mol) showcased the highest binding affinity against Me col-1. A glasshouse experiment was conducted to study the nematode suppressive effect of cell-free culture filtrate extracts of S. rochei GA, Streptomyces spp. GHS-3 and GHRS-5 on guava seedlings inoculated with M. enterolobii. Guava plants treated with S. rochei GA suppressed M. enterolobii parasitism with a 79.1% reduction in the number of egg masses, and promoted plant growth by 75%. This study highlights the nematotoxic potential of biomolecules produced by S. rochei GA as a promising alternative to synthetic nematicides for the management of M. enterolobii.

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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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