{"title":"芙蓉水提物对氯化镉致雄性Wistar大鼠神经毒性的影响。","authors":"Ogadinma Ilochi, Arthur N Chuemere","doi":"10.54548/njps.v39i1.14","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the neurologic effect of aqueous extract of Hibiscus sabdariffa on cadmium chloride-induced neurotoxicity in wistar rats. Thirty male wistar rats were grouped accordingly; Group A: control, Group B-F: CdCl2 0.3ml; H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 1.0ml; CdCl2 + H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 2ml, respectively. Gas chromatography coupled to flame ionization detector (GC-FID), total antioxidant capacity (TAC) and mineral analysis of H. sabdariffa was done to reveal the bioactive agents in the plant sample. Oxidative stress, muscle function markers and plasma electrolytes were assayed. Phytochemical screening revealed alkaloids, phytate, anthraquinone and flavonoids. The mineral analysis revealed predominantly, Mn, Ca, K and Fe. The biochemical results were in comparison with control and statistically significant at 95% confidence interval. There was an increase in SOD, CAT, GSH, GR and GPx in groups C-F, MDA decreased in group C, E and F while PC decreased in group C and F. There was a decrease in plasma creatine kinase in groups C to F and increase in AChE in group C. Ca and K levels had no significant change (P<0.05). Nitric oxide level had no significant change in group C, E and F, but increased in group D. This study demonstrated that H. sabdariffa has the potential to ameliorate the neurotoxicity caused by CdCl2 in wistar rats.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"39 1","pages":"111-118"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Aqueous Extract of Hibiscus Sabdariffa on Cadmium Chloride-Induced Neurotoxicity in Male Wistar Rats.\",\"authors\":\"Ogadinma Ilochi, Arthur N Chuemere\",\"doi\":\"10.54548/njps.v39i1.14\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the neurologic effect of aqueous extract of Hibiscus sabdariffa on cadmium chloride-induced neurotoxicity in wistar rats. Thirty male wistar rats were grouped accordingly; Group A: control, Group B-F: CdCl2 0.3ml; H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 1.0ml; CdCl2 + H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 2ml, respectively. Gas chromatography coupled to flame ionization detector (GC-FID), total antioxidant capacity (TAC) and mineral analysis of H. sabdariffa was done to reveal the bioactive agents in the plant sample. Oxidative stress, muscle function markers and plasma electrolytes were assayed. Phytochemical screening revealed alkaloids, phytate, anthraquinone and flavonoids. The mineral analysis revealed predominantly, Mn, Ca, K and Fe. The biochemical results were in comparison with control and statistically significant at 95% confidence interval. There was an increase in SOD, CAT, GSH, GR and GPx in groups C-F, MDA decreased in group C, E and F while PC decreased in group C and F. There was a decrease in plasma creatine kinase in groups C to F and increase in AChE in group C. Ca and K levels had no significant change (P<0.05). Nitric oxide level had no significant change in group C, E and F, but increased in group D. This study demonstrated that H. sabdariffa has the potential to ameliorate the neurotoxicity caused by CdCl2 in wistar rats.</p>\",\"PeriodicalId\":35043,\"journal\":{\"name\":\"Nigerian Journal of Physiological Sciences\",\"volume\":\"39 1\",\"pages\":\"111-118\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nigerian Journal of Physiological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.54548/njps.v39i1.14\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nigerian Journal of Physiological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54548/njps.v39i1.14","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
本研究探讨了芙蓉水提物对氯化镉致wistar大鼠神经毒性的影响。30只雄性wistar大鼠依次分组;A组:对照组,B-F组:CdCl2 0.3ml;H. sabdariffa 1.5ml;CdCl2 + H. sabdariffa 1.0ml;CdCl2 + H. sabdariffa 1.5ml;CdCl2 + H. sabdariffa 2ml。采用气相色谱联用火焰离子化检测器(GC-FID)、总抗氧化能力(TAC)和矿物分析等方法,对药材样品中的生物活性成分进行分析。测定氧化应激、肌肉功能指标及血浆电解质。植物化学筛选显示生物碱、植酸盐、蒽醌和类黄酮。矿物分析主要为Mn、Ca、K和Fe。生化结果与对照组比较,在95%置信区间具有统计学意义。C-F组SOD、CAT、GSH、GR、GPx升高,C、E、F组MDA降低,C、F组PC降低,C ~ F组血浆肌酸激酶降低,C组AChE升高,Ca、K水平无显著变化(P < 0.05)
Effect of Aqueous Extract of Hibiscus Sabdariffa on Cadmium Chloride-Induced Neurotoxicity in Male Wistar Rats.
This study investigated the neurologic effect of aqueous extract of Hibiscus sabdariffa on cadmium chloride-induced neurotoxicity in wistar rats. Thirty male wistar rats were grouped accordingly; Group A: control, Group B-F: CdCl2 0.3ml; H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 1.0ml; CdCl2 + H. sabdariffa 1.5ml; CdCl2 + H. sabdariffa 2ml, respectively. Gas chromatography coupled to flame ionization detector (GC-FID), total antioxidant capacity (TAC) and mineral analysis of H. sabdariffa was done to reveal the bioactive agents in the plant sample. Oxidative stress, muscle function markers and plasma electrolytes were assayed. Phytochemical screening revealed alkaloids, phytate, anthraquinone and flavonoids. The mineral analysis revealed predominantly, Mn, Ca, K and Fe. The biochemical results were in comparison with control and statistically significant at 95% confidence interval. There was an increase in SOD, CAT, GSH, GR and GPx in groups C-F, MDA decreased in group C, E and F while PC decreased in group C and F. There was a decrease in plasma creatine kinase in groups C to F and increase in AChE in group C. Ca and K levels had no significant change (P<0.05). Nitric oxide level had no significant change in group C, E and F, but increased in group D. This study demonstrated that H. sabdariffa has the potential to ameliorate the neurotoxicity caused by CdCl2 in wistar rats.