富含碳水化合物、蛋白质和脂肪的饮食对大鼠醋酸性胃溃疡愈合的年龄相关影响

Q4 Medicine
Uwaifoh Akpamu, Adeola T Salami, Olugbenga A Odukanmi, Samuel B Olaleye
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引用次数: 0

摘要

在这项研究中,我们比较了富含碳水化合物、蛋白质和脂肪的饮食对年轻和老年大鼠醋酸诱发溃疡的愈合效果。雄性Wistar大鼠(分别为3、6、12和18个月大,各40只)被分成4组,分别接受基础饮食(对照)、碳水化合物、蛋白质或脂肪丰富的饮食,在醋酸致胃损伤前21天。之后分别饲喂3 d和7 d。采用平面测量法进行溃疡愈合研究。我们用紫外/可见分光光度计测定氧化还原状态、胃蛋白酶、粘蛋白和一氧化氮活性,用免疫组织化学测定表皮生长因子受体(EGF-R)。数据分析采用双因素方差分析(two-way ANOVA), p≤0.05。第7天的溃疡愈合百分比相对于第3天随着年龄的增长而下降,但在喂食富含蛋白质的饮食的老年大鼠中增加。随着年龄的增长,胃羰基、丙二醛和胃蛋白酶活性显著升高,超氧化物歧化酶、过氧化氢酶、粘蛋白、一氧化氮和EGF-R表达显著降低。富含蛋白质的饮食调节了与年龄相关的变化。这些发现表明,富含蛋白质的饮食通过增强胃保护活性,促进溃疡胃中EGF-R的表达,从而促进醋酸诱导的胃损伤的愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Age-Related Effects of Carbohydrate-, Protein- And Fat-Rich Diets on Healing of Acetic Acid-Induced Gastric Ulcers in Rats.

In this study, we compared the ulcer healing effect of carbohydrate-, protein- and fat-rich diets on acetic acid-induced ulcers in young and aged rats. Male Wistar rats (40 each at 3-, 6-, 12-, and 18 months old) were grouped into four to receive basal diet (control), carbohydrate-, protein-, or fat-rich diets for 21 days before acetic acid-induced gastric injury. After this, the various feedings continued for 3- and 7 days. Planimetry was used for the ulcer healing study. We estimated the redox status, pepsin, mucin, and nitric oxide activities by UV/Vis-spectrophotometer while the Epidermal Growth Factor-Receptor (EGF-R) was by immunohistochemistry. Data was analyzed (two-way ANOVA) and was considered significant at p≤0.05. Percentage ulcer healing by day 7 relative to day 3 decreased with advancing age in other diets but increased in the aged rats fed a protein-rich diet. Gastric carbonyl, Malondehyde, and pepsin activities increased significantly with age, while superoxide dismutase, catalase, mucin, Nitric-oxide and EGF-R expression significantly decreased with age. Protein-rich diets modulated the age-related alterations. These findings suggest that a protein-rich diet facilitates the healing of acetic acid-induced gastric injury by enhancing gastroprotective activity to favour EGF-R expression in the ulcerated stomach.

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来源期刊
Nigerian Journal of Physiological Sciences
Nigerian Journal of Physiological Sciences Medicine-Physiology (medical)
CiteScore
0.80
自引率
0.00%
发文量
23
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