用于体外供体肺康复的血清疗法方法。

IF 12.8 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Med Pub Date : 2025-03-24 DOI:10.1016/j.medj.2025.100644
Meghan R Pinezich, John D O'Neill, Brandon A Guenthart, Jinho Kim, Olaia F Vila, Stephen P Ma, Ya-Wen Chen, Ahmed E Hozain, Aravind Krishnan, Moeed Fawad, Katherine M Cunningham, Holly M Wobma, Julie Van Hassel, Hans-Willem Snoeck, Matthew Bacchetta, Gordana Vunjak-Novakovic
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引用次数: 0

摘要

背景:约80%的供体肺不用于移植。体外肺与支持猪的交叉循环使最初被认为不适合移植的供体肺得以康复。体外肺康复需要强有力的治疗和诊断模式;然而,没有标准化的“治疗”方法被报道。方法:离体肺(n = 23;17例多灶性挫伤(n = 6,人)、胃误吸损伤(n = 8,猪)、缺血再灌注损伤(n = 3,猪)或无损伤(n = 6,猪)的受伤患者和6例对照组被用于制定一种交叉循环过程中体外肺康复的治疗和诊断(治疗)方法。气道(支气管镜,雾化),血管内和经胸膜通路允许样本收集和治疗递送。诊断方法包括无创成像、功能检测和分子分析。治疗方式包括支气管肺泡灌洗、表面活性剂替代、招募机动和细胞/类器官输送。通过荧光和生物发光成像对递送细胞进行实时跟踪。结果:诊断评估揭示了体外肺在交叉循环过程中整体和区域尺度的组织、细胞和分子水平的见解,这为治疗管理和干预提供了信息,以恢复供体肺。间充质间质细胞和肺类器官经支气管镜和胸膜输送,在交叉循环过程中无创追踪,并观察到在实质内定位。结论:在交叉循环过程中应用一种治疗方法,可以对各种肺损伤进行实时离体肺评估和康复,有助于提高将来对供体肺的临床利用。资助:NIH (P41 EB027062, R01HL120046, U01HL134760), CFF (VUNJAK23XX0)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Theranostic methodology for ex vivo donor lung rehabilitation.

Background: About 80% of donor lungs are not utilized for transplantation. Cross-circulation of ex vivo lungs with a support swine enables the rehabilitation of donor lungs that are initially deemed unsuitable for transplantation. Robust therapeutic and diagnostic modalities are needed for ex vivo lung rehabilitation; however, no standardized "theranostic" methodology has been reported.

Methods: Ex vivo lungs (n = 23; 17 injured and 6 controls) with multi-focal contusion (n = 6, human), gastric aspiration injury (n = 8, swine), ischemia-reperfusion injury (n = 3, swine), or no injury (n = 6, swine) were used to develop a therapeutic and diagnostic (theranostic) methodology for ex vivo lung rehabilitation during cross-circulation. Airway (bronchoscopic, nebulized), intravascular, and transpleural access enabled sample collection and therapeutic delivery. Diagnostic modalities included non-invasive imaging, functional testing, and molecular assays. Therapeutic modalities included bronchoalveolar lavage, surfactant replacement, recruitment maneuvers, and cell/organoid delivery. Real-time tracking of delivered cells was performed via fluorescence and bioluminescence imaging.

Findings: Diagnostic assessments revealed tissue-, cell-, and molecular-level insights at global and regional scales of ex vivo lungs during cross-circulation, which informed therapeutic management and interventions to recover donor lungs. Mesenchymal stromal cells and lung organoids were delivered bronchoscopically and transpleurally, tracked non-invasively during cross-circulation, and observed to localize within the parenchyma.

Conclusions: Application of a theranostic methodology during cross-circulation enabled real-time ex vivo lung assessment and rehabilitation across a variety of lung injuries to help increase clinical utilization of donor lungs in the future.

Funding: NIH (P41 EB027062, R01HL120046, U01HL134760), CFF (VUNJAK23XX0).

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来源期刊
Med
Med MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
17.70
自引率
0.60%
发文量
102
期刊介绍: Med is a flagship medical journal published monthly by Cell Press, the global publisher of trusted and authoritative science journals including Cell, Cancer Cell, and Cell Reports Medicine. Our mission is to advance clinical research and practice by providing a communication forum for the publication of clinical trial results, innovative observations from longitudinal cohorts, and pioneering discoveries about disease mechanisms. The journal also encourages thought-leadership discussions among biomedical researchers, physicians, and other health scientists and stakeholders. Our goal is to improve health worldwide sustainably and ethically. Med publishes rigorously vetted original research and cutting-edge review and perspective articles on critical health issues globally and regionally. Our research section covers clinical case reports, first-in-human studies, large-scale clinical trials, population-based studies, as well as translational research work with the potential to change the course of medical research and improve clinical practice.
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