Hongxiang Mei, Cai Qi, Jinchi Liu, Sirui Yang, Jiajia Zhou, Yangyu Lu, Liwei Zheng, Juan Li, Lei Zhao, Xin Xu
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Salivary microbiota was further analyzed and its correlation with hTAS2R38 polymorphisms was investigated.</p><p><strong>Results: </strong>In the cross-sectional study, a higher prevalence of halitosis was observed in individuals with AVI/AVI genotype as compared to non-AVI/AVI genotype (PAV/PAV + PAV/AVI) (OR = 2.380, 95% CI = 1.493-3.807). 16S rRNA sequencing revealed the enrichment of Prevotella in the saliva of AVI/AVI individuals. In the prospective study, halitosis patients with AVI/AVI genotype exhibited poor treatment outcome relative to non-AVI/AVI individuals during the 6-month follow-up after halitosis intervention (RR = 2.077, 95% CI = 1.382-3.339). Tannerella, Filifactor, and Mycoplasma were identified to be the major persistent genus in the saliva of AVI/AVI patients over the 6-month period after treatment. Furthermore, the human gingival fibroblasts with AVI/AVI genotype exhibited reduced inhibition against the growth and volatile sulfur compounds production of periodontal pathogens.</p><p><strong>Conclusions: </strong>Our work demonstrates that hTAS2R38 polymorphisms contribute to the development and treatment outcome of halitosis via modulating oral microbiota, providing new insights to the better management of halitosis. 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引用次数: 0
摘要
背景：口臭的遗传易感性尚不清楚，对高危人群的精确治疗提出了挑战。在这里，我们研究了hTAS2R38多态性与口臭患病率和治疗结果的关系，特别关注口腔微生物群。方法：对689名参与者进行横断面研究，探讨hTAS2R38基因多态性与口臭的关系。为探讨hTAS2R38基因多态性与口臭治疗效果的关系，建立了一项包括60例口臭患者的为期6个月的队列研究。进一步分析唾液微生物群，并探讨其与hTAS2R38多态性的相关性。结果：在横断面研究中，AVI/AVI基因型个体的口臭患病率高于非AVI/AVI基因型个体（PAV/PAV + PAV/AVI） （OR = 2.380, 95% CI = 1.493-3.807）。16S rRNA测序结果显示，AVI/AVI患者唾液中富含普雷沃氏菌。前瞻性研究中，在口臭干预后6个月的随访中，AVI/AVI基因型的口臭患者治疗效果较非AVI/AVI个体差（RR = 2.077, 95% CI = 1.382 ~ 3.339）。治疗后6个月，AVI/AVI患者唾液中主要存在单宁菌、丝状因子和支原体。此外，具有AVI/AVI基因型的人牙龈成纤维细胞对牙周病原体生长和挥发性硫化合物产生的抑制作用降低。结论：我们的研究表明，hTAS2R38基因多态性通过调节口腔微生物群参与了口臭的发生和治疗结果，为更好地管理口臭提供了新的见解。视频摘要。
hTAS2R38 polymorphisms modulate oral microbiota and influence the prevalence and treatment outcome of halitosis.
Background: The genetic susceptibility of halitosis is unclear, challenging its precise management in high-risk population. Here we investigated the association of hTAS2R38 polymorphisms with the prevalence and treatment outcome of halitosis, with a particular focus on oral microbiota.
Methods: A cross-sectional study including 689 participants was conducted to investigate the association between hTAS2R38 polymorphisms and halitosis. A 6-month cohort including 60 halitosis patients was established to explore the relationship between hTAS2R38 polymorphisms and treatment outcome of halitosis. Salivary microbiota was further analyzed and its correlation with hTAS2R38 polymorphisms was investigated.
Results: In the cross-sectional study, a higher prevalence of halitosis was observed in individuals with AVI/AVI genotype as compared to non-AVI/AVI genotype (PAV/PAV + PAV/AVI) (OR = 2.380, 95% CI = 1.493-3.807). 16S rRNA sequencing revealed the enrichment of Prevotella in the saliva of AVI/AVI individuals. In the prospective study, halitosis patients with AVI/AVI genotype exhibited poor treatment outcome relative to non-AVI/AVI individuals during the 6-month follow-up after halitosis intervention (RR = 2.077, 95% CI = 1.382-3.339). Tannerella, Filifactor, and Mycoplasma were identified to be the major persistent genus in the saliva of AVI/AVI patients over the 6-month period after treatment. Furthermore, the human gingival fibroblasts with AVI/AVI genotype exhibited reduced inhibition against the growth and volatile sulfur compounds production of periodontal pathogens.
Conclusions: Our work demonstrates that hTAS2R38 polymorphisms contribute to the development and treatment outcome of halitosis via modulating oral microbiota, providing new insights to the better management of halitosis. Video Abstract.
期刊介绍：
Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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