改良技术在1厘米芯针活检中使用2厘米切割长度的同轴引入针的疗效。

IF 2.2 3区 医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Hiroki Satomura, Yasushi Kimura, Masahisa Nakamura, Kosuke Tomotake, Koki Yamamoto, Kaishu Tanaka, Yusuke Ono, Hiroki Higashihara, Noriyuki Tomiyama
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引用次数: 0

摘要

目的:本研究评估了使用2厘米行程长度的改良核心针活检来提高癌症基因组医学分子分析的组织产量的有效性。方法:使用不同硬度的琼脂糖凝胶进行体外活检重量评估。用鸡肝、鸡胗、鸡胸和猪腰组织进行离体活检体重评估。此外,采用小鼠皮下肿瘤模型进行了体内实验。改进的方法包括使用同轴引入针来维持1厘米的缺口长度,同时采用2厘米的行程长度来增加组织产量。活检性能参数,包括样品重量和DNA产率,比较改进和传统方法。使用高速摄像机测量1 cm和2 cm行程长度活检针的射速。结果:在所有琼脂糖凝胶浓度下,改进的技术显著增加了样品体积(增加9.5-13%)。结论:改进的活检技术使用2cm的行程长度显著提高了组织产量,提高了精确医学分子分析中活检样品的质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacy of modified technique for 1 cm core needle biopsy using a 2 cm cutting length with coaxial introducer needle

Purpose

This study evaluated the efficacy of a modified core needle biopsy using a 2 cm stroke length to improve tissue yield for molecular analyses in cancer genomics medicine.

Methods

In vitro biopsy weight evaluations were performed using agarose gels of varying hardness. Ex vivo biopsy weight evaluations were conducted using chicken liver, gizzard, breast, and pork loin tissues. Furthermore, in vivo experiments were performed using murine subcutaneous tumor models. The modified method involved using a coaxial introducer needle to maintain a 1 cm notch length while employing a 2 cm stroke length to increase tissue yield. Biopsy performance parameters, including sample weight and DNA yield, were compared between the modified and conventional methods. The firing speed of the biopsy needles with 1 cm and 2 cm stroke lengths was also measured using a high-speed camera.

Results

The modified technique significantly increased the sample volume across all agarose gel concentrations (9.5–13% increase, P < 0.05). Ex vivo tests revealed significantly higher tissue yields in most samples, except for chicken gizzards. In vivo, the modified method produced significantly larger tissue samples (4.6 mg vs. 2.9 mg, P < 0.001) and higher DNA yield (8.2 ng vs. 6.9 ng, P < 0.05). In addition, the biopsy needle’s firing speed was 1.4–1.8 times faster with the 2 cm stroke length than 1 cm stroke.

Conclusion

The modified biopsy technique using a 2 cm stroke length significantly improved tissue yield, enhancing the quality of biopsy samples for molecular analyses in precision medicine.

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来源期刊
Abdominal Radiology
Abdominal Radiology Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
5.20
自引率
8.30%
发文量
334
期刊介绍: Abdominal Radiology seeks to meet the professional needs of the abdominal radiologist by publishing clinically pertinent original, review and practice related articles on the gastrointestinal and genitourinary tracts and abdominal interventional and radiologic procedures. Case reports are generally not accepted unless they are the first report of a new disease or condition, or part of a special solicited section. Reasons to Publish Your Article in Abdominal Radiology: · Official journal of the Society of Abdominal Radiology (SAR) · Published in Cooperation with: European Society of Gastrointestinal and Abdominal Radiology (ESGAR) European Society of Urogenital Radiology (ESUR) Asian Society of Abdominal Radiology (ASAR) · Efficient handling and Expeditious review · Author feedback is provided in a mentoring style · Global readership · Readers can earn CME credits
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