血小板源性生长因子- bb对人下颌骨髓源性间充质干细胞生物活性影响的评价

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-03-26 DOI:10.1016/j.archoralbio.2025.106244

Yurika Oura, Masakazu Ishii, Haruka Miyata, Nao Ikeda, Tomoaki Sakurai, Fumio Suehiro, Naohiro Komabashiri, Masahiro Nishimura

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引用次数: 0

摘要

目的本研究旨在探讨血小板衍生生长因子-BB（PDGF-BB）对人下颌骨骨髓间充质干细胞（MBMSCs）生物活性的影响。设计用血小板衍生生长因子-BB（20 ng/mL）处理MBMSCs，并评估其对细胞增殖、成骨分化和迁移的影响。细胞增殖采用 WST-1 试验进行评估。成骨分化通过测量矿化电位和碱性磷酸酶活性进行评估。细胞迁移是通过伤口愈合和 Transwell 室试验进行评估的。使用免疫荧光染色法评估细胞骨架重组和粘附动态。使用 Western 印迹法评估了 PDGF-BB 刺激诱导的 MBMSCs 细胞内信号的变化。此外，我们还研究了调节 PDGF-BB 诱导的细胞迁移的分子机制--Girdin 信号转导。PDGF-BB促进了MBMSCs的迁移。PDGF-BB处理增强了F-肌动蛋白丝的形成和paxillin在细胞前缘的定位。PDGF-BB 可激活 MBMSCs 中的 Akt 信号转导，抑制 Akt 信号转导可有效抑制 PDGF-BB 诱导的 Akt 激活和迁移。PDGF-BB 促进了 MBMSCs 中 Girdin 的磷酸化，抑制 Akt 信号转导可减轻 PDGF-BB 诱导的 Girdin 激活。此外，PDGF-BB 诱导的 MBMSCs 迁移可能是通过 Akt/Girdin 信号通路介导的。这些发现为我们深入了解 PDGF-BB 诱导牙周组织再生的分子机制提供了重要依据。

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Evaluation of the effect of platelet-derived growth factor-BB on the biological activity of human mandibular bone marrow-derived mesenchymal stem cells

Objective

This study aimed to investigate the effects of platelet-derived growth factor-BB (PDGF-BB) on the biological activities of human mandibular bone marrow-derived mesenchymal stem cells (MBMSCs).

Design

PDGF-BB (20 ng/mL) was used to treat MBMSCs, and its effects on their proliferation, osteogenic differentiation, and migration were evaluated. Cell proliferation was evaluated using a WST-1 assay. Osteogenic differentiation was evaluated by measuring the mineralization potential and alkaline phosphatase activity. Cell migration was evaluated using wound healing and Transwell chamber assays. Cytoskeletal reorganization and adhesion dynamics were evaluated using immunofluorescence staining. Changes in intracellular signaling in MBMSCs induced by PDGF-BB stimulation were evaluated using western blotting. Furthermore, we investigated Girdin signaling as the molecular mechanisms underlying the regulation of PDGF-BB-induced cell migration.

Results

PDGF-BB treatment did not affect the proliferation or osteogenic differentiation of MBMSCs. PDGF-BB promoted the migration of MBMSCs. PDGF-BB treatment enhanced F-actin filament formation and paxillin localization at the leading edge of cells. PDGF-BB treatment activated Akt signaling in MBMSCs, and the inhibition of Akt signaling effectively suppressed PDGF-BB-induced Akt activation and migration. PDGF-BB promoted the phosphorylation of Girdin in MBMSCs, and the inhibition of Akt signaling attenuated PDGF-BB-induced Girdin activation.

Conclusion

This study demonstrated that PDGF-BB strongly induces the migration of MBMSCs without affecting their proliferation or osteogenic differentiation. Furthermore, PDGF-BB-induced migration of MBMSCs may be mediated through the Akt/Girdin signaling pathway. These findings provide important insight into the molecular mechanisms underlying PDGF-BB-induced periodontal tissue regeneration.

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry