转录因子FOXD3通过调控nAChR-5参与马氏平锥虫的免疫应答

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-03-26 DOI:10.1016/j.aquaculture.2025.742486

Min Yang , Yongge Huang , Xinyi Lin , Zhihan Li , Shilin Zheng , Youmei Qu , Yue Wen Deng , Ronglian Huang , Yu Jiao

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引用次数: 0

摘要

马氏珠贝是一种重要的海水珍珠牡蛎，需要进行移植手术才能生产珍珠。然而，移植手术经常引起炎症反应，导致免疫排斥或珍珠牡蛎死亡。我们的前期研究阐明了马氏体烟碱乙酰胆碱受体5 （PmnAChR-5）在移植免疫中的重要作用。在本研究中，我们获得了PmnAChR-5基因编码序列上游的2000 bp，并利用双荧光素酶报告系统验证了其高启动子活性。叉头转录因子D3 （PmFOXD3）被预测为PmnAChR-5的关键调节因子。双荧光素酶报告基因实验表明，过表达PmFOXD3显著增强PmnAChR-5启动子活性。电泳迁移迁移试验（EMSA）证实了PmFOXD3在PmnAChR-5启动子区的结合位点（TTTTGTTTGTT）。转录组学分析显示，PmFOXD3表达下调导致c型凝集素受体和Ras信号通路等通路显著富集，通路相关基因下调，血清中TNF-α水平降低。这些发现提示PmFOXD3通过调节PmnAChR-5启动子活性参与免疫应答。我们的研究结果为减轻珍珠生产过程中的免疫排斥反应提供了新的分子靶点。

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Transcription factor FOXD3 participates in the immune response of Pinctada fucata martensii by regulating nAChR-5

Pinctada fucata martensii, a significant seawater pearl oyster, requires transplantation surgery for pearl production. However, transplantation surgery often induces inflammatory responses, leading to immune rejection or mortality of the pearl oysters. Our previous research elucidated the crucial role of nicotinic acetylcholine receptor 5 from P. f. martensii (PmnAChR-5) in the transplantation immunity. In this study, we obtained the 2000 bp upstream of the coding sequences of PmnAChR-5 genes and verified its highly promoter activity using the dual-luciferase reporter system. Forkhead transcription factor D3 (PmFOXD3) was predicted as a key regulator of PmnAChR-5. Dual luciferase reporter assay demonstrated that overexpression of PmFOXD3 significantly enhanced the promoter activity of PmnAChR-5. Electrophoretic mobility shift assay (EMSA) verified the binding site (TTTTGTTTGTT) of PmFOXD3 in the promoter region of PmnAChR-5. Transcriptomic analysis revealed that down-regulated expression of PmFOXD3 lead to significant enrichment in pathways such as C-type lectin receptor and Ras signaling pathways, along with knockdown of pathway-related genes and decreased TNF-α levels in serum. These findings suggested that PmFOXD3 is involved in the immune response by regulating the promoter activity of PmnAChR-5. Our results provide novel molecular targets for mitigating immune rejection during pearl production.

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.