Xingyue Fan , Yue Sun , Jiaqi Fu , Hui Cao , Shiyi Liao , Cheng Zhang , Shuangyan Huan , Guosheng Song
{"title":"磁共振响应纳米探针用于观察糖尿病肝损伤中的过氧化氢","authors":"Xingyue Fan , Yue Sun , Jiaqi Fu , Hui Cao , Shiyi Liao , Cheng Zhang , Shuangyan Huan , Guosheng Song","doi":"10.1016/j.biomaterials.2025.123292","DOIUrl":null,"url":null,"abstract":"<div><div>Diabetic liver injury has emerged as a significant complication associated with diabetes, warranting increased attention. The generation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) due to oxidative stress plays a critical role in the onset and progression of this condition. Despite this, there is a scarcity of probes capable of non-invasively, accurately, and reliably visualizing H<sub>2</sub>O<sub>2</sub> levels in deep-seated liver in diabetes-induced liver injury. In this study, we introduce a novel H<sub>2</sub>O<sub>2</sub>-responsive magnetic probe (H<sub>2</sub>O<sub>2</sub>-RMP), designed for the sensitive imaging of H<sub>2</sub>O<sub>2</sub> in the liver injury caused by diabetes. H<sub>2</sub>O<sub>2</sub>-RMP is synthesized through the co-precipitation of a H<sub>2</sub>O<sub>2</sub>-responsive amphiphilic polymer, manganese(III) porphyrin (Mn-porphyrin), and iron oxide nanoparticles. When exposed to H<sub>2</sub>O<sub>2</sub>, the released iron oxide nanoparticles aggregate, resulting in an increased T<sub>2</sub>-weighted MR signal intensity. H<sub>2</sub>O<sub>2</sub>-RMP not only demonstrates a wide dynamic response range (initial r<sub>2</sub> = 9.87 mM<sup>-</sup><sup>1</sup>s<sup>-</sup><sup>1</sup>, Δr<sub>2</sub> = 7.69 mM<sup>-</sup><sup>1</sup>s<sup>-</sup><sup>1</sup>), but also exhibits superior selectivity for H<sub>2</sub>O<sub>2</sub> compared to other reactive oxygen species. Importantly, H<sub>2</sub>O<sub>2</sub>-RMP exhibits high sensitivity, with a detection limit for hydrogen peroxide as low as 0.56 μM. Moreover, H<sub>2</sub>O<sub>2</sub>-RMP has been effectively applied for real-time imaging of H<sub>2</sub>O<sub>2</sub> levels in the livers of diabetic model mice with varying degrees of severity, highlighting its potential for visual diagnosis and monitoring the progression of diabetic liver injury.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"321 ","pages":"Article 123292"},"PeriodicalIF":12.8000,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"MRI-responsive nanoprobes for visualizing hydrogen peroxide in diabetic liver injury\",\"authors\":\"Xingyue Fan , Yue Sun , Jiaqi Fu , Hui Cao , Shiyi Liao , Cheng Zhang , Shuangyan Huan , Guosheng Song\",\"doi\":\"10.1016/j.biomaterials.2025.123292\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Diabetic liver injury has emerged as a significant complication associated with diabetes, warranting increased attention. The generation of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) due to oxidative stress plays a critical role in the onset and progression of this condition. Despite this, there is a scarcity of probes capable of non-invasively, accurately, and reliably visualizing H<sub>2</sub>O<sub>2</sub> levels in deep-seated liver in diabetes-induced liver injury. In this study, we introduce a novel H<sub>2</sub>O<sub>2</sub>-responsive magnetic probe (H<sub>2</sub>O<sub>2</sub>-RMP), designed for the sensitive imaging of H<sub>2</sub>O<sub>2</sub> in the liver injury caused by diabetes. H<sub>2</sub>O<sub>2</sub>-RMP is synthesized through the co-precipitation of a H<sub>2</sub>O<sub>2</sub>-responsive amphiphilic polymer, manganese(III) porphyrin (Mn-porphyrin), and iron oxide nanoparticles. When exposed to H<sub>2</sub>O<sub>2</sub>, the released iron oxide nanoparticles aggregate, resulting in an increased T<sub>2</sub>-weighted MR signal intensity. H<sub>2</sub>O<sub>2</sub>-RMP not only demonstrates a wide dynamic response range (initial r<sub>2</sub> = 9.87 mM<sup>-</sup><sup>1</sup>s<sup>-</sup><sup>1</sup>, Δr<sub>2</sub> = 7.69 mM<sup>-</sup><sup>1</sup>s<sup>-</sup><sup>1</sup>), but also exhibits superior selectivity for H<sub>2</sub>O<sub>2</sub> compared to other reactive oxygen species. Importantly, H<sub>2</sub>O<sub>2</sub>-RMP exhibits high sensitivity, with a detection limit for hydrogen peroxide as low as 0.56 μM. Moreover, H<sub>2</sub>O<sub>2</sub>-RMP has been effectively applied for real-time imaging of H<sub>2</sub>O<sub>2</sub> levels in the livers of diabetic model mice with varying degrees of severity, highlighting its potential for visual diagnosis and monitoring the progression of diabetic liver injury.</div></div>\",\"PeriodicalId\":254,\"journal\":{\"name\":\"Biomaterials\",\"volume\":\"321 \",\"pages\":\"Article 123292\"},\"PeriodicalIF\":12.8000,\"publicationDate\":\"2025-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomaterials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S014296122500211X\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S014296122500211X","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
MRI-responsive nanoprobes for visualizing hydrogen peroxide in diabetic liver injury
Diabetic liver injury has emerged as a significant complication associated with diabetes, warranting increased attention. The generation of hydrogen peroxide (H2O2) due to oxidative stress plays a critical role in the onset and progression of this condition. Despite this, there is a scarcity of probes capable of non-invasively, accurately, and reliably visualizing H2O2 levels in deep-seated liver in diabetes-induced liver injury. In this study, we introduce a novel H2O2-responsive magnetic probe (H2O2-RMP), designed for the sensitive imaging of H2O2 in the liver injury caused by diabetes. H2O2-RMP is synthesized through the co-precipitation of a H2O2-responsive amphiphilic polymer, manganese(III) porphyrin (Mn-porphyrin), and iron oxide nanoparticles. When exposed to H2O2, the released iron oxide nanoparticles aggregate, resulting in an increased T2-weighted MR signal intensity. H2O2-RMP not only demonstrates a wide dynamic response range (initial r2 = 9.87 mM-1s-1, Δr2 = 7.69 mM-1s-1), but also exhibits superior selectivity for H2O2 compared to other reactive oxygen species. Importantly, H2O2-RMP exhibits high sensitivity, with a detection limit for hydrogen peroxide as low as 0.56 μM. Moreover, H2O2-RMP has been effectively applied for real-time imaging of H2O2 levels in the livers of diabetic model mice with varying degrees of severity, highlighting its potential for visual diagnosis and monitoring the progression of diabetic liver injury.
期刊介绍:
Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice. The scope of the journal covers the wide range of physical, biological and chemical sciences that underpin the design of biomaterials and the clinical disciplines in which they are used. These sciences include polymer synthesis and characterization, drug and gene vector design, the biology of the host response, immunology and toxicology and self assembly at the nanoscale. Clinical applications include the therapies of medical technology and regenerative medicine in all clinical disciplines, and diagnostic systems that reply on innovative contrast and sensing agents. The journal is relevant to areas such as cancer diagnosis and therapy, implantable devices, drug delivery systems, gene vectors, bionanotechnology and tissue engineering.