揭示miRNA在喉鳞癌进展中的作用：一项回顾性研究。

IF 1.9 3区医学 Q2 OTORHINOLARYNGOLOGY

European Archives of Oto-Rhino-Laryngology Pub Date : 2025-03-28 DOI:10.1007/s00405-025-09306-y

Nejmiye Akkuş, Elif Kaya Çelik, Suleyman Ozdemir, Emrah Sapmaz

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引用次数: 0

摘要

目的：MicroRNAs （miRNAs）已成为喉鳞癌（LSCC）诊断和预后的有前途的生物标志物。本研究探讨了早期和晚期LSCC中特定癌症相关mirna的表达模式，以评估其在疾病进展中的潜在作用。方法：采用定量多重RT-PCR方法分析44例LSCC患者（早期20例，晚期24例）福尔马林固定石蜡包埋（FFPE）组织中miRNA-21、miRNA-802、miRNA-29和miRNA-9的表达水平。回顾性分析组织样本。结果：晚期患者hsa-miR-9a表达水平较早期组显著上调（5.2倍）（p = 0.0088）。同样，hsa-miR-802a在晚期患者中的表达量明显高于早期患者（9.2倍）（p = 0.005）。各组间miRNA-21和miRNA-29表达水平无显著差异。结论：研究结果表明，晚期患者miRNA水平升高可用于靶向治疗，并可作为LSCC患者的一种治疗选择进行评估。

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Unveiling the role of miRNA in laryngeal squamous cell carcinoma progression: a retrospective study.

Objective: MicroRNAs (miRNAs) have emerged as promising biomarkers for diagnosis and prognosis in laryngeal squamous cell carcinoma (LSCC). This study investigates the expression patterns of specific cancer-associated miRNAs in early and advanced stage LSCC to evaluate their potential role in disease progression.

Methods: The expression levels of miRNA-21, miRNA-802, miRNA-29 and miRNA-9 were analysed in formalin-fixed paraffin-embedded (FFPE) tissues from 44 LSCC patients (20 early stage, 24 advanced stage) using quantitative multiplex RT-PCR. Tissue samples were analysed retrospectively.

Results: The expression level of hsa-miR-9a expression was significantly upregulated (5.2-fold) in advanced stage patients compared to the early stage group (p = 0.0088). Similarly, hsa-miR-802a showed significantly higher expression (9.2-fold) in advanced stage patients compared to early stage patients (p = 0.005). No significant differences in miRNA-21 and miRNA-29 expression levels were observed between the groups.

Conclusion: As a result of the study, elevated miRNA levels in advanced stage patients can be used in targeted therapy and evaluated as a treatment option in LSCC patients.

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来源期刊

European Archives of Oto-Rhino-Laryngology 医学-耳鼻喉科学

CiteScore

5.30

自引率

7.70%

发文量

537

审稿时长

2-4 weeks

期刊介绍： Official Journal of European Union of Medical Specialists – ORL Section and Board Official Journal of Confederation of European Oto-Rhino-Laryngology Head and Neck Surgery "European Archives of Oto-Rhino-Laryngology" publishes original clinical reports and clinically relevant experimental studies, as well as short communications presenting new results of special interest. With peer review by a respected international editorial board and prompt English-language publication, the journal provides rapid dissemination of information by authors from around the world. This particular feature makes it the journal of choice for readers who want to be informed about the continuing state of the art concerning basic sciences and the diagnosis and management of diseases of the head and neck on an international level. European Archives of Oto-Rhino-Laryngology was founded in 1864 as "Archiv für Ohrenheilkunde" by A. von Tröltsch, A. Politzer and H. Schwartze.