基于中毒性弥漫性甲状腺肿（Graves病）患者甲状腺组织和全血mRNA的促甲状腺素受体胞外结构域的表达

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI:10.1007/s10517-025-06351-9

A V Zubkov, L G Butova, N S Kuzmina, I V Zubkova, V V Fadeev, O A Svitich

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引用次数: 0

摘要

基于从甲状腺组织和中毒性弥漫性甲状腺肿（Graves病）患者全血中分离的mRNA，克隆了甲状腺自身免疫性疾病（Graves病和桥本甲状腺炎）自身抗体结合位点对应的胞外结构域432-和389-bp区域的促甲状腺素受体基因（TSHR）片段。在18例患者中，6例患者检测到432 bp的片段，3例患者检测到389 bp的片段。在一名患者中，两种碎片都从血液中分离出来。结果表明，30%的Graves病患者血液中有TSHR表达。

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Expression of the Extracellular Domain of the Thyrotropin Receptor Based on mRNA Isolated from Thyroid Tissue and Whole Blood of Patients with Toxic Diffuse Goiter (Graves' Disease).

Fragments of the thyrotropin receptor gene (TSHR) encoding 432- and 389-bp regions of the extracellular domain corresponding to the autoantibody binding sites in autoimmune diseases of the thyroid gland (Graves' disease and Hashimoto's thyroiditis, respectively) were cloned based on mRNA isolated not only from the thyroid tissue, but also from the whole blood of patients with toxic diffuse goiter (Graves' disease). In a group of 18 patients, the 432-bp fragment was detected in 6 patients and the 389-bp fragment was detected in 3 patients. In one patient, both fragments were isolated from the blood. The results indicate that TSHR is expressed in the blood in 30% of patients with Graves' disease.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.