衰老影响小鼠视网膜氧和血流指标

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-03-26 DOI:10.1016/j.exer.2025.110363

Farzan Abdolahi , Mansour Rahimi , Rutuja Zinjal , Sava Sakadzic , Mahnaz Shahidi

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引用次数: 0

摘要

老化影响眼睛，是眼部疾病的主要危险因素。本研究的目的是确定年龄对小鼠视网膜氧和血流指标的影响。方法将47只C57BL/6小鼠分为4个年龄组（3月龄(m): N = 18, 9月龄：N = 13, 15月龄：N = 8, 21月龄：N = 8），采用建立的多模态成像系统测量视网膜动脉和静脉直径（DA， DV），静脉血流速（VV），动脉和静脉氧含量（O2A, O2V）。计算平均血流量（BF）、氧输送（DO2）、代谢（MO2）和提取分数（OEF）。采用方差分析和事后分析来确定年龄对变量的影响。结果VV、BF、O2A、O2V、DO2在不同年龄组间存在显著差异。与3m组相比，VV在21m组显著降低（p = 0.004），在15m组略有降低（p = 0.06），分别降低了41%和30%。21m组BF低于3m组（p = 0.007），降低42%。此外，与9m组相比，21m组O2A和O2V显著降低（p≤0.05），分别降低30%和65%。相比于15m组（p = 0.05）和9m组（p = 0.04）， 21m组DO2显著降低，分别降低46%和44%。MO2、OEF、DA、DV差异无统计学意义（p≥0.07）。结论老龄小鼠视网膜血管含氧量、供氧率和血流量明显降低，建立了按年龄划分的视网膜生理生物标志物基线。

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Aging affects retinal oxygen and blood flow metrics in mice

Purpose

Aging affects the eyes and is a major risk factor for ocular diseases. The purpose of this study was to determine the effect of age on retinal oxygen and blood flow metrics in mice.

Methods

Forty-seven C57BL/6 mice were included in four age groups (3-month-old (m): N = 18, 9m: N = 13, 15m: N = 8, and 21m: N = 8). Retinal arterial and venous diameters (D_A, D_V), venous blood velocity (V_V), and arterial and venous oxygen contents (O_2A, O_2V) were measured by our established multimodal imaging system. Average blood flow (BF), oxygen delivery (DO₂), metabolism (MO₂), and extraction fraction (OEF) were calculated. ANOVA, and Tukey post-hoc analyses were performed to determine the effect of age on variables.

Results

We found significant differences in V_V, BF, O_2A, O_2V, and DO₂ among the age groups. V_V was significantly lower in the 21m (p = 0.004) and marginally lower in 15m (p = 0.06) compared to the 3m group, representing reductions of 41% and 30%, respectively. BF was lower in the 21m than the 3m group (p = 0.007), a 42% reduction. Additionally, O_2A and O_2V were significantly lower in 21m compared to the 9m group (p ≤ 0.05), indicating reductions of 30% and 65%, respectively. DO₂ was significantly decreased in the 21m compared to 15m (p = 0.05) and 9m (p = 0.04) groups, representing reductions of 46% and 44%, respectively. Differences in MO₂, OEF, D_A, and D_V did not reach statistical significance (p ≥ 0.07).

Conclusions

Our results showed that retinal vascular oxygen content, oxygen delivery rate, and blood flow were significantly reduced in older mice and established baselines for retinal physiological biomarkers according to age.

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.