Human tissue oxygen and strontium isotope values in North America: A data compilation and assessment for forensic geolocation.

The use of isotopic analysis for human mobility, geolocation, and forensic identification has become common over the past two decades, yet its effectiveness depends on the availability of well-documented reference data. Many reference data exist in the literature, but the suitability of these data for forensic applications has not been critically assessed. Here, we compile oxygen and strontium isotope data for North American human tissues (hair, nails, bone, and tooth enamel). We review the geographic distribution of these data, evaluate their relationship with the predicted geographic variation, and assess potential sources of bias that may limit the comparability of different datasets in the compilation. A substantial number of data are available for some of these substrates and isotope systems, but in most cases, their geographic distribution is patchy with many areas under sampled. Except for hair Sr isotopes, a robust relationship occurs between human tissue values and modeled local environmental values, suggesting theoretically expected relationships between human tissues and local sources of O and Sr are expressed. These relationships are noisy; we identify several methodological differences that produce systematic biases across the compiled data. Based on these findings, we suggest interpreting forensic isotope data using currently published reference data is problematic. We propose the adoption of consistent metadata reporting and standardized laboratory protocols to enhance the utility of data gathered in future research as these practices could lead to measurable improvements in the strength of forensic interpretations derived from human tissue isotope data.