生成纤维化小鼠精确肺切片的优化方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-06-20 Epub Date: 2025-03-24 DOI:10.1016/j.xpro.2025.103711

Stefanos Smyrniotis, Paraskevi Kanellopoulou, Christiana Magkrioti, Vassilis Aidinis

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引用次数: 0

摘要

与体内动物模型和体外细胞模型相比，精确切割肺切片（pcls）具有显著的优势，使其成为呼吸研究的宝贵工具。在这里，我们提出了一种生成纤维化小鼠pcls （mpcls）的方案，它提供了与人类pcls相同的益处，但绕过了人类组织可用性和相关伦理问题，同时也增强了实验的同质性和可重复性。该方案详细介绍了博来霉素（BLM）诱导的肺纤维化、PCLS生成、可视化和下游分析的步骤。有关本协议使用和执行的完整细节，请参阅Barbayianni等人1。

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An optimized protocol for generating fibrotic murine precision-cut lung slices.

Precision-cut lung slices (PCLSs) offer significant advantages over both in vivo animal models and in vitro cell models, making them invaluable tools in respiratory research. Here, we present a protocol for generating fibrotic mouse PCLSs (mPCLSs), which offer the same benefits as human PCLSs but bypass issues of human tissue availability and related ethical concerns while also enhancing experimental homogeneity and reproducibility. This protocol details the steps for bleomycin (BLM)-induced pulmonary fibrosis, PCLS generation, visualization, and downstream analysis. For complete details on the use and execution of this protocol, please refer to Barbayianni et al.¹.

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