Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao
{"title":"鸡布鲁氏菌候选疫苗株(abcBA)在小鼠感染布鲁氏菌模型中的聚合佐剂评价","authors":"Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao","doi":"10.12834/VetIt.3016.31419.2","DOIUrl":null,"url":null,"abstract":"<p><p>Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 3","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of polymeric adjuvants associated with cadidate vaccine strain Brucella ovis ∆abcBA in a murine model of Brucella ovis infection.\",\"authors\":\"Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao\",\"doi\":\"10.12834/VetIt.3016.31419.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.</p>\",\"PeriodicalId\":23550,\"journal\":{\"name\":\"Veterinaria italiana\",\"volume\":\"60 3\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2024-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinaria italiana\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.12834/VetIt.3016.31419.2\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria italiana","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.12834/VetIt.3016.31419.2","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Evaluation of polymeric adjuvants associated with cadidate vaccine strain Brucella ovis ∆abcBA in a murine model of Brucella ovis infection.
Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.
期刊介绍:
The journal was created as the Croce Azzurra in 1950.
A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy.
The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.