鸡布鲁氏菌候选疫苗株(abcBA)在小鼠感染布鲁氏菌模型中的聚合佐剂评价

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES
Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao
{"title":"鸡布鲁氏菌候选疫苗株(abcBA)在小鼠感染布鲁氏菌模型中的聚合佐剂评价","authors":"Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao","doi":"10.12834/VetIt.3016.31419.2","DOIUrl":null,"url":null,"abstract":"<p><p>Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"60 3","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of polymeric adjuvants associated with cadidate vaccine strain Brucella ovis ∆abcBA in a murine model of Brucella ovis infection.\",\"authors\":\"Jefferson Brunno Soares Oliveira, Fabiola Barroso Costa, Pamela Aparecida Lima, Thaynara Parente de Carvalho, Monique Ferreira Silva, Laice Alves da Silva, Michelle Daiane de Almeida Loures, Humberto De Mello Brandão, Renato De Lima Santos, Tatiane Alves Paixao\",\"doi\":\"10.12834/VetIt.3016.31419.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.</p>\",\"PeriodicalId\":23550,\"journal\":{\"name\":\"Veterinaria italiana\",\"volume\":\"60 3\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2024-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinaria italiana\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.12834/VetIt.3016.31419.2\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria italiana","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.12834/VetIt.3016.31419.2","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

布鲁氏菌病是一种由兼性胞内革兰氏阴性菌引起的传染病,对动物和人类健康具有重要意义。一种理想的布鲁氏菌病疫苗应能产生保护作用,不会在动物或人类中引起疾病,并且不会干扰血清学诊断。疫苗佐剂可以改善免疫反应,导致更强烈和更持久的保护,提高其有效性。鹅布鲁氏菌ΔabcBA菌株封装在海藻酸盐提供了一种实验性疫苗配方,防止布鲁氏菌感染。然而,其他聚合物如佐剂的研究对于优化候选疫苗B. ovis∆abcBA的效率是重要的。因此,本研究旨在评估不同聚合佐剂联合的鹅弧菌∆abcBA在鹅弧菌攻毒小鼠中的疫苗潜力。我们观察到,与未接种疫苗的小鼠相比,被海藻酸盐和壳聚糖包裹的B. ovis∆abcBA,而不是共聚物Poloxamer 407,导致小鼠脾脏和肝脏的细菌回收率最低。虽然共聚物poloxam407没有诱导显著的体液免疫应答,但海藻酸盐和壳聚糖疫苗制剂诱导了更高水平的免疫球蛋白G,并增加了IgG2b亚类,表明Th1型应答,已知Th1型应答在控制细胞内病原体感染中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of polymeric adjuvants associated with cadidate vaccine strain Brucella ovis ∆abcBA in a murine model of Brucella ovis infection.

Brucellosis is an infectious disease caused by facultative intracellular Gram-negative bacteria, of great importance in animal and human health. An ideal vaccine against brucellosis should induce protection, not cause disease in animals or humans, and not interfere with serological diagnosis. Vaccine adjuvants can improve the immune response, leading to a more intense and prolonged protection, improving its effectiveness. The Brucella ovis ΔabcBA strain encapsulated in alginate provides an experimental vaccine formulation that protects against Brucella spp. infection. However, the investigation of other polymers such as adjuvants is important for optimizing the efficiency of the candidate vaccine B. ovis ∆abcBA. Therefore, this study aimed to evaluate the vaccine potential of the B. ovis ∆abcBA associated with different polymeric adjuvants in mice challenged with B. ovis. We observed that B. ovis ∆abcBA encapsulated by alginate with chitosan, but not copolymer Poloxamer 407, resulted in the lowest bacterial recovery in both the spleen and liver of challenged animals compared to non-vaccinated mice. While copolymer Poloxamer 407 did not induce significant humoral immune response, the alginate and chitosan vaccine formulation induced higher levels of Immunoglobulin G, with an increase in the IgG2b subclass, indicating a Th1 type of response, which is known to play a critical role in controlling infections by intracellular agents.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信