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IF 1.8 3区医学 Q2 PARASITOLOGY

Parasitology Research Pub Date : 2025-03-26 DOI:10.1007/s00436-025-08482-4

Milena Camargo, Laura Vega, Marina Muñoz, Lauren Hernández-Buelvas, Leidy Pedraza, Indira M Báez-Murcia, Juan David Ramírez, Manuel Alfonso Patarroyo

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引用次数: 0

摘要

阴道毛滴虫是一种影响人类生殖健康的鞭毛原虫。虽然细菌合并感染与阴道毛滴虫感染结果有关，但相关数据却很少。本研究旨在评估患者随访期间阴道细菌群落与阴道球菌感染结果（感染、清除、持续存在）有关的变化。这项为期一年的随访研究涉及哥伦比亚妇女，她们提供了两次宫颈细胞学刮片（一次在基线时，另一次在随访时），平均随访间隔为 12 ± 1 个月。阴道球菌通过传统 PCR（Tvk3/7 和 BTU9/2 引物）检测。评估微生物群组成时使用了 Illumina Novaseq PE250 平台。这项研究涉及 66 名妇女（132 份样本）；68.2%（n = 45）的妇女在基线时阴性，31.8%（n = 21）的妇女被初步诊断为阴道蓟马感染（99 304 个扩增子序列变异被鉴定/归类为 62 个门和 1908 个属）。清除了阴道特异性淋病菌的妇女（10 人）显示出微生物丰富度的增加，而阴道特异性淋病菌持续存在的妇女（11 人）则显示出更高的微生物多样性。在菌属相对丰度方面观察到了显著的变化：清除组的乳酸杆菌丰度增加（p = 0.002），持续感染组的普雷沃特氏菌丰度增加（p = 0.045），获得性阴道杆菌感染组的加德纳菌、Megasphaera 和 Sneathia 丰度增加（p = 0.045）。在相对丰度方面，逻辑回归分析表明，阴道特异性阴道炎持续存在的妇女中，Sneathia 的丰度呈上升趋势（调整 OR 为 2.23），乳酸杆菌的丰度呈下降趋势（调整 OR 为 0.39）。DESeq 分析显示，在随访期间未感染的妇女中，鸟疫菌属大幅减少，而在出现各种阴道特异性感染结果的妇女中，反刍球菌增加。研究结果表明，阴道假丝酵母菌感染期间存在的特定细菌属可能会影响其清除率，这对改善诊断和治疗策略以促进妇女生殖健康具有潜在的意义。

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Unveiling shifts in cervical microbiota composition among Colombian women in the presence of Trichomonas vaginalis: a longitudinal study.

Trichomonas vaginalis is a flagellated protozoan affecting human reproductive health. Although bacterial coinfections have been associated with T. vaginalis infection outcomes, pertinent data are scarce. This study was aimed at assessing vaginal bacterial community changes regarding T. vaginalis outcomes (infection, clearance, persistence) during patients' follow-up visits. This was a 1-year follow-up study involving Colombian women who provided two cytological scrapings from the cervix (one at baseline and another at follow-up), having an average 12 ± 1 month follow-up interval. T. vaginalis was detected by conventional PCR (Tvk3/7 and BTU9/2 primers). The Illumina Novaseq PE250-platform was used for assessing microbiota composition. This study involved 66 women (132 samples); 68.2% (n = 45) tested negative for T. vaginalis at baseline while 31.8% (n = 21) were initially diagnosed with T. vaginalis infection (99,304 amplicon sequence variants identified/categorised into 62 phyla and 1908 genera). Women who cleared T. vaginalis (n = 10) displayed increased microbial richness, while those having persistence (n = 11) had higher microbial diversity. Significant changes were observed regarding genus relative abundance: Lactobacillus abundance increased in the clearance group (p = 0.002), Prevotella in the persistence group (p = 0.045) and Gardnerella, Megasphaera and Sneathia in the group having acquired T. vaginalis infection (p = 0.045). Regarding relative abundance, logistic regression analysis revealed a positive trend concerning Sneathia increase (adjusted OR 2.23) and reduced Lactobacillus abundance (adjusted OR 0.39) in women with T. vaginalis persistence. DESeq analysis revealed a substantial decrease in the Ornithinimicrobium genus among women without infection during follow-up, while Ruminococcus increased in women having various T. vaginalis outcomes. The results suggested that specific bacterial genera present during T. vaginalis infection may influence its clearance, having potential implications for improving diagnostic and therapeutic strategies for enhancing women's reproductive health.

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来源期刊

Parasitology Research 医学-寄生虫学

CiteScore

4.10

自引率

5.00%

发文量

346

审稿时长

6 months

期刊介绍： The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.