Vineeta V Khanvilkar, Shruti S Mandle, Prachi R Hande, Bhavin R Daka, Pratik P Jadhav
{"title":"反相高效液相色谱法测定制剂中小檗碱和熊果酸含量的建立及验证。","authors":"Vineeta V Khanvilkar, Shruti S Mandle, Prachi R Hande, Bhavin R Daka, Pratik P Jadhav","doi":"10.1093/chromsci/bmaf016","DOIUrl":null,"url":null,"abstract":"<p><p>Medicines used in ayurveda are typically made of many herbs or are polyherbals. Standardization of these medicines can be carried out by quantitative estimation of two or more of its markers simultaneously. Berberine and ursolic acid are two active markers which are present in an Ayurvedic formulation, Giloy Tulsi tablets. In the present research work, the Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the simultaneous quantitative determination of berberine and ursolic acid from the formulation. With an RP Hemochrom C18 column, chromatographic separation was accomplished at 292 nm with eluent composed of methanol: acetonitrile (4:1, v/v) at a flow rate of 1.0 mL/min. The retention time of berberine and ursolic acid was obtained to be 1.5 ± 0.02 and 3.2 ± 0.02 min, respectively. A simple, rapid, accurate, specific, and robust analytical method was developed to estimate berberine and ursolic acid. This validated method can be used for routine quality control of ayurvedic formulations or herbal formulations having plants which contain berberine and ursolic acid.</p>","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 4","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"RP-HPLC Method Development and Validation for Simultaneous Estimation of Berberine and Ursolic Acid in Formulation.\",\"authors\":\"Vineeta V Khanvilkar, Shruti S Mandle, Prachi R Hande, Bhavin R Daka, Pratik P Jadhav\",\"doi\":\"10.1093/chromsci/bmaf016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Medicines used in ayurveda are typically made of many herbs or are polyherbals. Standardization of these medicines can be carried out by quantitative estimation of two or more of its markers simultaneously. Berberine and ursolic acid are two active markers which are present in an Ayurvedic formulation, Giloy Tulsi tablets. In the present research work, the Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the simultaneous quantitative determination of berberine and ursolic acid from the formulation. With an RP Hemochrom C18 column, chromatographic separation was accomplished at 292 nm with eluent composed of methanol: acetonitrile (4:1, v/v) at a flow rate of 1.0 mL/min. The retention time of berberine and ursolic acid was obtained to be 1.5 ± 0.02 and 3.2 ± 0.02 min, respectively. A simple, rapid, accurate, specific, and robust analytical method was developed to estimate berberine and ursolic acid. This validated method can be used for routine quality control of ayurvedic formulations or herbal formulations having plants which contain berberine and ursolic acid.</p>\",\"PeriodicalId\":15430,\"journal\":{\"name\":\"Journal of chromatographic science\",\"volume\":\"63 4\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of chromatographic science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1093/chromsci/bmaf016\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatographic science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1093/chromsci/bmaf016","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
RP-HPLC Method Development and Validation for Simultaneous Estimation of Berberine and Ursolic Acid in Formulation.
Medicines used in ayurveda are typically made of many herbs or are polyherbals. Standardization of these medicines can be carried out by quantitative estimation of two or more of its markers simultaneously. Berberine and ursolic acid are two active markers which are present in an Ayurvedic formulation, Giloy Tulsi tablets. In the present research work, the Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the simultaneous quantitative determination of berberine and ursolic acid from the formulation. With an RP Hemochrom C18 column, chromatographic separation was accomplished at 292 nm with eluent composed of methanol: acetonitrile (4:1, v/v) at a flow rate of 1.0 mL/min. The retention time of berberine and ursolic acid was obtained to be 1.5 ± 0.02 and 3.2 ± 0.02 min, respectively. A simple, rapid, accurate, specific, and robust analytical method was developed to estimate berberine and ursolic acid. This validated method can be used for routine quality control of ayurvedic formulations or herbal formulations having plants which contain berberine and ursolic acid.
期刊介绍：
The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
