内镜下缓解的日本溃疡性结肠炎患者肠道吞噬体的特征。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2024-11-20 DOI:10.3164/jcbn.24-173
Akinori Otsuki, Ryo Inoue, Takayuki Imai, Hiroto Miura, Atsushi Nishida, Osamu Inatomi, Akira Andoh
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引用次数: 0

摘要

本研究旨在分析内镜下缓解的日本溃疡性结肠炎(UC)患者的肠道噬菌体。收集35例UC患者和22例健康对照者的粪便样本。采用16S rRNA扩增子测序分析肠道微生物组,采用散弹枪宏基因组测序分析噬菌体。与健康对照组相比,UC患者的噬菌体丰富度(观察物种和Chao1指数)显著降低。主坐标分析显示UC与健康对照之间β -多样性有显著差异(p = 0.001)。UC中温带噬菌体的丰度(15.2%)高于健康对照组(5.9%),尽管这没有统计学意义(p = 0.088)。UC患者中与Coprococcus sp., Bacteroides sp. KFT8, Faecalibacterium prausnitzii相关的温带噬菌体,以及与Ruminococcus gnavus和Lactobacillus farciminis相关的强毒噬菌体增加。相反,UC患者中与影响热吸菌、OF03-11BH类拟杆菌和恶臭杆菌相关的噬菌体减少。UC患者中与气味杆菌属(p = 0.0004)、瘤胃球菌属(p = 0.009)和细孔菌属(p = 0.013)相关的噬菌体显著减少。与健康对照组相比,非活动性UC患者的肠道噬菌体表现出明显的病毒组成改变。这些结果提示肠道噬菌体的改变可能参与了UC的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of the gut phageome of Japanese patients with ulcerative colitis under endoscopic remission.

This study aimed to analyze the gut phageome in Japanese patients with ulcerative colitis (UC) in endoscopic remission. Fecal samples were collected from 35 UC patients and 22 healthy controls. The gut microbiome was analyzed using 16S rRNA amplicon sequencing, and the phageome was profiled through shotgun metagenomic sequencing. Compared to healthy controls, UC patients showed a significant reduction in phageome richness (observed species and Chao1 index). Principal coordinate analysis revealed a significant difference in beta-diversity between UC and healthy controls (p = 0.001). The abundance of temperate phages was higher in UC (15.2%) compared to healthy controls (5.9%), although this was not statistically significant (p = 0.088). Temperate phages associated with Coprococcus sp., Bacteroides sp. KFT8, and Faecalibacterium prausnitzii, as well as virulent phages associated with Ruminococcus gnavus and Lactobacillus farciminis, were increased in UC patients. Conversely, phages associated with Thermosipho affectus, Bacteroides sp. OF03-11BH, and Odoribacter splanchnicus were decreased in UC patients. Phages associated with the genera Odoribacter (p = 0.0004), Ruminococcus (p = 0.009), and Veillonella (p = 0.013) were significantly reduced in UC patients. The gut phageome of inactive UC patients exhibited notable alterations in viral composition compared to healthy controls. These results suggest that changes in the gut phageome might be involved in the pathogenesis of UC.

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来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
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