利用CRISPR/Cas9技术在人iPSCs中培养ABCA7敲除纯合子细胞系AHMUCNi002-A

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-03-25 DOI:10.1016/j.scr.2025.103700

Juanjuan Li , Letian Yin , Chengwei Wang , Yin Xu

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引用次数: 0

摘要

ABCA7位于19号染色体上，编码atp结合盒转运体。ABCA7的功能丧失变体与阿尔茨海默病的风险增加有关。为了探索ABCA7缺失在阿尔茨海默病发病机制中的作用，利用CRISPR/Cas9基因组编辑技术在人诱导多能干细胞（hiPSCs）中产生ABCA7纯合子敲除。由此产生的ABCA7敲除细胞系表现出正常的多能性、稳定的核型和向所有三种胚层分化的能力。

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Generation of a homozygous ABCA7 knockout cell line (AHMUCNi002-A) in human iPSCs using CRISPR/Cas9

ABCA7, located on chromosome 19, encodes an ATP-binding cassette transporter. Loss-of-function variants of ABCA7 are associated with an increased risk of Alzheimer’s disease. To explore the role of ABCA7 deficiency in the pathogenesis of Alzheimer’s disease, CRISPR/Cas9 genome-editing technology was utilized to generate a homozygous ABCA7 knockout in human induced pluripotent stem cells (hiPSCs). The resulting ABCA7 knockout cell line exhibited normal pluripotency, a stable karyotype, and the ability to differentiate into all three germ layers.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.