C57BL/6N小鼠肝损伤及抑郁样行为海马小胶质细胞形态学差异

Current health sciences journal Pub Date : 2024-10-01 Epub Date: 2024-12-31 DOI:10.12865/CHSJ.50.04.12
Gabriel Nedelea, Mădălina Iuliana Mușat, Smaranda Ioana Mitran, Mihai Călin Ciorbagiu, Bogdan Cătălin
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引用次数: 0

摘要

小胶质细胞是中枢神经系统最重要的细胞之一,随着病理的不同而发生特异性的变化。据报道,抑郁症和肝脏疾病都会引起海马的变化和神经炎症。然而,这两种病理对海马小胶质细胞形态的综合影响尚未得到充分的探讨。材料和方法:本研究采用共聚焦显微镜和半人工定量方法分析海马小胶质细胞的形态学变化。我们关注的是总分支长度、分支数和平均分支长度。使用C57BL/6N小鼠,并对其进行蛋氨酸和胆碱缺乏饮食(MCD)诱导肝损伤,以及慢性不可预测的轻度应激(CUMS)程序,以诱导抑郁样行为。结果:我们能够证明,CUMS方案和MCD饮食导致总分支长度、分支数量和平均分支长度的减少。此外,单独的CUMS与次级和终末分支数量的减少有关。结论:我们的研究表明,抑郁样行为和肝损伤影响海马小胶质细胞形态,这可能在未来对这些复杂病理的研究中得到考虑。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphological Differences in Hippocampal Microglia in C57BL/6N Mice with Liver Injury and Depressive-Like Behavior.

Introduction: Microglia, one of the most important cells of the central nervous system, undergo specific changes depending on the pathology. It has been reported that both depressive disorders and liver diseases generate hippocampal changes and neuroinflammation. However, the combined effects of the two pathologies on microglia morphology in the hippocampus have not been sufficiently explored.

Material and methods: In this study, we analyzed the morphological changes of the hippocampal microglia using confocal microscopy and a semi-manual method of quantification. We focused on total branch length, the branch number and the mean branch length. C57BL/6N mice were used and subjected to a methionine and choline deficient diet (MCD) to induce liver damage, and a chronic unpredictable mild stress (CUMS) procedure for depressive-like behavior.

Results: We were able to show that CUMS protocol and MCD diet led to a reduction in total branch length, branch number and mean branch length. Also, CUMS alone was associated with a decrease in the number of secondary and terminal branches.

Conclusion: Our study showed that depressive-like behavior and liver damage influence microglial morphology in the hippocampus, and it may be considered in future research of these intricate pathologies.

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