人参皂苷Rg6通过抑制聚焦和诱导自噬提高卵巢癌上皮细胞对顺铂的耐药性。

IF 5.5
The American journal of Chinese medicine Pub Date : 2025-01-01 Epub Date: 2025-03-27 DOI:10.1142/S0192415X25500247
Kai Xue, Yun Bai, Yufei Han, Chuanxiang Yao, Zhenzhe Zhao, Dongyang Liang, Feiyu Lu, Yinping Jin, Jiazhe Song
{"title":"人参皂苷Rg6通过抑制聚焦和诱导自噬提高卵巢癌上皮细胞对顺铂的耐药性。","authors":"Kai Xue, Yun Bai, Yufei Han, Chuanxiang Yao, Zhenzhe Zhao, Dongyang Liang, Feiyu Lu, Yinping Jin, Jiazhe Song","doi":"10.1142/S0192415X25500247","DOIUrl":null,"url":null,"abstract":"<p><p>Platinum-based chemotherapy remains a mainstay of clinical practice in the standard treatment of epithelial ovarian cancer (EOC). Most patients who receive this treatment, however, develop relapse and drug resistance. Ginsenoside Rg6 (G-Rg6), one of the anticarcinogenic active components in the American ginseng berry, may hold promise in the adjuvant chemotherapy of EOC. In this study, the correlation between fucosylation and cisplatin (cDDP) resistance in EOC cells was validated by gene expression profile analysis and lectin blot. We found that G-Rg6 derived from the American ginseng berry inhibits the cell viability and protein fucosylation of cDDP-resistant EOC cells. G-Rg6-induced G<sub>2</sub>/M-cell cycle arrest was proven to result from the autophagy of cDDP-resistant EOC cells. In addition, we observed that G-Rg6 initiates autophagy in cDDP-resistant EOC cells by inhibiting the GRB2-ERK1/2-mTOR axis, and that high concentration of G-Rg6 treatment leads to cell apoptosis. G-Rg6 also enhances cDDP uptake in A2780CP cells by promoting CTR1 expression and suppressing its core fucosylation. Therapies combining cDDP and G-Rg6 display higher efficacy in inhibiting the cDDP-resistant EOC cells in comparison with the sole application of cDDP, exhibiting strong potential for clinical application. G-Rg6 derived from the American ginseng berry can improve cDDP resistance in EOC cells via suppressing fucosylation and inducing autophagy, suggesting its potential in the adjuvant chemotherapy of EOC patients.</p>","PeriodicalId":94221,"journal":{"name":"The American journal of Chinese medicine","volume":" ","pages":"621-646"},"PeriodicalIF":5.5000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ginsenoside Rg6 Improves Cisplatin Resistance in Epithelial Ovarian Cancer Cells via Suppressing Fucosylation and Inducing Autophagy.\",\"authors\":\"Kai Xue, Yun Bai, Yufei Han, Chuanxiang Yao, Zhenzhe Zhao, Dongyang Liang, Feiyu Lu, Yinping Jin, Jiazhe Song\",\"doi\":\"10.1142/S0192415X25500247\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Platinum-based chemotherapy remains a mainstay of clinical practice in the standard treatment of epithelial ovarian cancer (EOC). Most patients who receive this treatment, however, develop relapse and drug resistance. Ginsenoside Rg6 (G-Rg6), one of the anticarcinogenic active components in the American ginseng berry, may hold promise in the adjuvant chemotherapy of EOC. In this study, the correlation between fucosylation and cisplatin (cDDP) resistance in EOC cells was validated by gene expression profile analysis and lectin blot. We found that G-Rg6 derived from the American ginseng berry inhibits the cell viability and protein fucosylation of cDDP-resistant EOC cells. G-Rg6-induced G<sub>2</sub>/M-cell cycle arrest was proven to result from the autophagy of cDDP-resistant EOC cells. In addition, we observed that G-Rg6 initiates autophagy in cDDP-resistant EOC cells by inhibiting the GRB2-ERK1/2-mTOR axis, and that high concentration of G-Rg6 treatment leads to cell apoptosis. G-Rg6 also enhances cDDP uptake in A2780CP cells by promoting CTR1 expression and suppressing its core fucosylation. Therapies combining cDDP and G-Rg6 display higher efficacy in inhibiting the cDDP-resistant EOC cells in comparison with the sole application of cDDP, exhibiting strong potential for clinical application. G-Rg6 derived from the American ginseng berry can improve cDDP resistance in EOC cells via suppressing fucosylation and inducing autophagy, suggesting its potential in the adjuvant chemotherapy of EOC patients.</p>\",\"PeriodicalId\":94221,\"journal\":{\"name\":\"The American journal of Chinese medicine\",\"volume\":\" \",\"pages\":\"621-646\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The American journal of Chinese medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1142/S0192415X25500247\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The American journal of Chinese medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1142/S0192415X25500247","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/27 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

以铂为基础的化疗仍然是上皮性卵巢癌(EOC)标准治疗的临床实践支柱。然而,大多数接受这种治疗的患者会复发并产生耐药性。人参皂苷Rg6 (G-Rg6)是西洋参果实中的一种抗癌活性成分,可能在EOC的辅助化疗中有应用前景。本研究通过基因表达谱分析和凝集素印迹验证了聚焦化与EOC细胞顺铂(cDDP)耐药之间的相关性。我们发现,从西洋参浆果中提取的G-Rg6可以抑制抗cddp的EOC细胞的细胞活力和蛋白聚焦。g - rg6诱导的G2/ m细胞周期阻滞是由抗cddp的EOC细胞自噬引起的。此外,我们观察到G-Rg6通过抑制GRB2-ERK1/2-mTOR轴在抗cddp的EOC细胞中启动自噬,高浓度G-Rg6处理导致细胞凋亡。G-Rg6也通过促进CTR1表达和抑制其核心聚焦而增强A2780CP细胞对cDDP的摄取。与单独应用cDDP相比,cDDP与G-Rg6联合治疗对cDDP耐药EOC细胞的抑制效果更高,具有较强的临床应用潜力。西洋参提取物G-Rg6可通过抑制聚焦和诱导自噬来改善EOC细胞对cDDP的耐药性,提示其在EOC患者辅助化疗中的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ginsenoside Rg6 Improves Cisplatin Resistance in Epithelial Ovarian Cancer Cells via Suppressing Fucosylation and Inducing Autophagy.

Platinum-based chemotherapy remains a mainstay of clinical practice in the standard treatment of epithelial ovarian cancer (EOC). Most patients who receive this treatment, however, develop relapse and drug resistance. Ginsenoside Rg6 (G-Rg6), one of the anticarcinogenic active components in the American ginseng berry, may hold promise in the adjuvant chemotherapy of EOC. In this study, the correlation between fucosylation and cisplatin (cDDP) resistance in EOC cells was validated by gene expression profile analysis and lectin blot. We found that G-Rg6 derived from the American ginseng berry inhibits the cell viability and protein fucosylation of cDDP-resistant EOC cells. G-Rg6-induced G2/M-cell cycle arrest was proven to result from the autophagy of cDDP-resistant EOC cells. In addition, we observed that G-Rg6 initiates autophagy in cDDP-resistant EOC cells by inhibiting the GRB2-ERK1/2-mTOR axis, and that high concentration of G-Rg6 treatment leads to cell apoptosis. G-Rg6 also enhances cDDP uptake in A2780CP cells by promoting CTR1 expression and suppressing its core fucosylation. Therapies combining cDDP and G-Rg6 display higher efficacy in inhibiting the cDDP-resistant EOC cells in comparison with the sole application of cDDP, exhibiting strong potential for clinical application. G-Rg6 derived from the American ginseng berry can improve cDDP resistance in EOC cells via suppressing fucosylation and inducing autophagy, suggesting its potential in the adjuvant chemotherapy of EOC patients.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信